Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1972-11-15
1976-01-06
Mack, John H.
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
204180S, 204299, B01D 1302
Patent
active
039309738
ABSTRACT:
A method and apparatus for electrophoresis, counter electrophoresis, or immuno electrophoresis on a cellulose acetate membrane carried out on a flat rigid support plate supported over buffer reservoirs of opposite polarity. The membrane is wetted with buffer solution and pressed against the plate to flatten it and excess buffer solution is removed. Wicks are placed at opposite edges of the membrane and extend into the buffer reservoirs. Multiple samples are applied to the membrane and are electrophoresed. Several membranes may be placed across the plate with interconnecting wicks for series electrophoresis. Or several membranes may be superposed on the plate with diffusion sample to the lowermost plate for parallel electrophoresis.
The invention also provides a system for rapid and accurate placement of samples to be tested on the membrane.
The method and apparatus in another embodiment are suitable for thin layer gel chromatography and electrophoresis.
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Heftmann, "Chromatography," 2nd Ed., pp. 255-259, 269 and 270.
Canley, "Electrophoresis and Immunoelectrophoresis," pp. 1, and 230-233, Little, Brown & Co., Boston, 1969.
Mack John H.
Prescott A. C.
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