Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1990-07-09
1993-06-01
Housel, James C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435287, 422 8208, 356344, 935 85, 935 77, 204299R, 2041801, 2041828, G12Q 168, C12M 100, G01N 2100
Patent
active
052158837
ABSTRACT:
A process and apparatus for detecting the mobility of fluorescently labeled molecules in response to a force is disclosed. The fluorescently labeled molecules are placed in a fluid medium such as an electrophoretic gel in a capillary tube. An electric field is applied to induce the movement of the molecules. A region of the labeled molecules is photobleached leaving a geometrically defined region which has not been photobleached. The region which has not been photobleached is excited by a reading beam. The reading and the photobleaching beams can be generated by focusing a laser through a diffraction grading. The intensity produced by the interaction of the reading beam in the geometrically defined region of the molecule is detected by a photo detector. In detecting more than one sp
REFERENCES:
patent: 4222744 (1980-09-01), McConnell
patent: 4675095 (1987-06-01), Kambara et al.
patent: 4832815 (1989-05-01), Kambara et al.
Chu et al., Biopolymers, 28, 1491-1494 (1989).
Wang et al., Physical Review Letters, 63 (22), 2528-2531 (1989).
Wu et al., Biopolymers, 29, 491-500 (1990).
Schwartz et al., Cell, 37, 67-75 (1984).
Carle et al., Science, 232, 65-68 (1986).
Cantor et al., Ann. Rev. Biophys. Chem., 17, 287-304 (1988).
Anand, Trends Genet., 2, 278-283 (1986).
Chu et al., Biopolymers, 27, 2005-2009 (1988).
Lerman et al., Biopolymers, 21, 995-997 (1982).
Lumpkin, et al., Biopolymers, 21, 2315-2316 (1982).
Lumpkin et al., Biopolymers, 24, 1573-1593 (1985).
Slater et al., Physical Review Letters, 55 (15) 1579-1582.
Slater et al., Biopolymers, 25, 431-454 (1986).
Noolandi et al., Phys. Rev. Lett., 58 (23), 2428-2431 (1987).
Hervet et al., Biopolymers, 26, 727-742 (1987).
Zimm, Phys. Rev. Lett., 61 (26), 2965-2968 (1988).
Noolandi et al., Science, 243, 1456-1458 (1989).
Deutsch, Science, 240, 922-924 (1988).
Deutsch, J. Chem. Phys., 90 (12), 7436-7441 (1989).
Deutsch et al., J. Chem. Phys., 90 (4), 2476-2485 (1989).
Holtzwarth et al., Nucleic Acids Res., 15, 10031-10044 (1987).
Smith et al., Science, 243, 203-206 (1989).
Schwartz et al., Letters to Nature, 338, 520-522 (1989).
Holtzwarth et al., Biopolymers, 28, 1043-1058 (1989).
Smith et al., Proc. Natl. Acad. Sci. USA, 75, 2759-2763 (1978).
Lanni & Ware, Rev. Sci. Instrum., 53 (6), 905-908 (1982).
Wahl, Biophys. Chem., 22, 317-321 (1985).
Peters et al., Biochim. Biophys. Acta, 367 282-294 (1974).
Axelrod et al., Biophys. J., 16 , 1055-1069 (1976).
Jacobson et al., Biochim. Biophys. Acta, 433, 215-222 (1976).
Koppel et al., Biophys. J., 16, 1315-1329 (1976).
Lanni et al., Biophys. J., 35, 351-364 (1981).
Kasper et al., J. Chromatogr., 458, 303-312 (1988).
Rhee et al., J. Phys. Chem., 88, 3944-3946 (1984).
Kim et al., J. Phys. Chem., 88, 3946-3949 (1984).
Fangman, Nucleic Acids Res., 5 (3), 653-665 (1978).
Slater et al., Biopolymers, 27, 509-524 (1988).
Righetti et al., Journal of Biochemical & Biophysical Methods, 4, 347-363 (1981).
Stellwagen, Biopolymers, 24, 2243-2255 (1985).
Serwer et al., Electrophoresis, 4, 273-276 (1983).
Serwer et al., Analytical Biochemistry, 158, 72-78 (1986).
Chan William K. Y.
Housel James C.
The Research Foundation
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