Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1987-09-22
1989-08-29
Schofer, Joseph L.
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
2041801, C25D 1300
Patent
active
048614483
ABSTRACT:
Gel inserts comprising a solidified liquid such as agarose suitable for use in an electrophoretic method, lysed cells entrapped within a matrix formed by the solidified liquid and macromolecules such as DNA or intact chromosomes derived from the lysed cells may be advantageously used in electrophoretic separations. The gel inserts are placed directly in a suitable support medium and subjected to one or more electric fields to separate the macromolecules.
REFERENCES:
patent: 4391688 (1983-07-01), Hamelin
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Romeyn, J. A., et al., "Anti-immunoglobulin analysis by diffusion patterns of inhibition and facilitation of complementary lysis in agar. I. Diffusion-lysis patterns of heterologous guinea pig anti-immunoglobulins," and II. Diffusion-lysis as a method for recognizing in vivo immunosuppressive activity of anti-immunoglobulins, J. Immunol. Meth., 6 pp. 363-374 & 375-384 (1975).
Gewurz, H., et al., "Complement," Manual of Clinical Immunology, 2d ed., Rose, N. R., et al., ed., Am. Soc. Microbiology, Washington, D.C., Section C, Chapter 19, pp. 163-174 (1980).
Garvey, J., et al., Methods in Immunology, 3d ed., pp. 411-424 (1977).
Ginsburg, W. W., et al., "Circulating and mitogen-induced immunoglobulin-secreting cells in human peripheral blood; evaluation by a modified reverse hemolytic plaque assay," The Journal of Immunology, 120(1): 33-39 (1978).
Eby, W. C., et al., "Enumerating immunoglobulin-secreting cells among peripheral human lymphocytes. A hemolytic plaque assay for a B cell function," The Journal of Immunology, 155(6): 1700-1703 (1975).
Cantor Charles R.
Schwartz David C.
Reddick J. M.
Schofer Joseph L.
The Trustees of Columbia University in the City of New York
White John P.
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