Electrophoretic apparatus for the quantitative elution of protei

Details Electrophoretic apparatus for the quantitative elution of protei Electrophoretic apparatus for the quantitative elution of protei

1982-11-18

1985-11-12

Metz, Andrew H.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

204299R, G01N 2728

Patent

active

045526400

ABSTRACT:
An apparatus for the quantitative elution of proteins or polypeptides from a gel by means of electrophoresis. The apparatus includes an upper chamber for holding a buffer solution containing the gel from which the proteins or polypeptides are to be eluted. An upper electrode is provided in the upper chamber. A lower chamber for holding a buffer solution is disposed beneath the upper chamber and includes a lower electrode. A septum separates the upper chamber from the lower chamber. A connecting passage in the septum connects the upper and lower chambers. A collecting capsule for the proteins or polypeptides is disposed at the end of the connecting passage in the lower chamber, and is adapted to be suspended in the buffer solution which is to be held in the lower chamber.

REFERENCES:
patent: 3533933 (1970-10-01), Strauch
patent: 3579433 (1971-05-01), Dahlgren
patent: 3773648 (1973-11-01), Van Welzen et al.
patent: 3980546 (1976-09-01), Caccavo
Laemmli, Nature, vol. 227, "Cleavage of Structural Proteins During the Asbly of the Head of Bacteriophage T4", pp. 680-685, 1970.
Jean O. Thomas et al, Proc. Nat. Acad. Sci. USA, vol. 72, No. 7, "An Octamer of Histones in Chromatin and Free in Solution", pp. 2626-2630, 1975.

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