Electrophoretic apparatus

Details Electrophoretic apparatus Electrophoretic apparatus

1993-05-25

1994-12-06

Niebling, John

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

B01D 6146

Patent

active

053707826

ABSTRACT:
An electophoretic apparatus for determining the base sequence of DNA has a device for introducing a sample, a device for controlling the temperature of the sample in the sample introducing device, a device for causing electrophoresis of the temperature-controlled sample in a gel to separate it into fragments, and a device for analyzing those fragments. The apparatus makes it possible to carry out successively and automatically a process which determines the base sequence of DNA by treating DNA in accordance with the Sanger method to form complementary DNA fragments of different lengths ending specifically with four different kinds of bases, separating those fragments from one another electrophoretically, and identifying the base at the end of each of those fragments.

REFERENCES:
Toneguzzo et al., A System for On-Line Detection and Resolution of Radiolabeled DNA Molecules and its Application to Automated DNA Sequence Analysis, Biotechniques, 7(8), 1989.
Voet and Voet, Biochemistry, pp. 830-835, 1990.

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