Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1991-10-04
1994-10-25
Niebling, John
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
204299R, G01N 2726, G01N 27447
Patent
active
053586123
ABSTRACT:
Improved capillary electrophoresis apparatus of the type that generally includes a capillary tube, the capillary tube having a sample inlet end and an outlet end, a first electrode in electrical communication with the inlet end of the capillary tube, a second electrode in electrical communication with the outlet end of the capillary tube and a high voltage power supply in electrical communication with the first and second electrodes. The improvement is to connect a miniature Ion Chromatography membrane suppressor to the outlet end of the capillary tube, to connect a conductivity detector to the membrane suppressor and to place the second electrode in the regenerant compartment of the membrane suppressor. The method embodiment of the invention for anion analysis includes the steps of: (a) separating anions of interest by capillary electrophoresis in a buffer solution; (b) exchanging cations of the buffer for regenerant cations using an ion chromatography membrane suppressor to reduce the electrical conductivity of the buffer to produce a suppressed buffer; and (c) measuring the electrical conductivity of the suppressed buffer to determine the separated anions. Similarly, the method embodiment of the invention for cation analysis includes the steps of: (a) separating cations of interest by capillary electrophoresis in a buffer solution; (b) exchanging anions of the buffer for regenerant anions using a stationary means for exchanging anions thereby reducing the electrical conductivity of the buffer to produce a suppressed buffer; and (c) measuring the electrical conductivity of the suppressed buffer to determine the separated cations.
REFERENCES:
patent: 4403039 (1983-09-01), Ban et al.
patent: 4453991 (1984-06-01), Grout
patent: 4459357 (1984-07-01), Jansen et al.
patent: 4474664 (1984-10-01), Stevens et al.
patent: 4500430 (1985-02-01), Dasgupta
patent: 4731263 (1988-03-01), Martin et al.
patent: 4751004 (1988-06-01), Stevens et al.
Journal of Chromatography, 593 (1992) 305-312, Elsevier Science Publishers B.V., Amsterdam, Capillary Electrophoresis with Electrochemical Detection Employing an On-Column Nafion Joint, Thomas J. O'Shea, et al.
Lunte, et al., Abstract, Poster Session 1, 15th Int. Symposium on Column Liquid Chromatography, Basel, Switzerland, Jun. 3-7, 1991.
F. M. Everaerts, et al., Journal of Chromatography Library, 22A, 1983, Elsevier Scientific Publishing Co.
J. W. Jorgenson and K. D. Lukasc, Zone Electrophoresis in Open-Tubular Glass Capillaries, Anal. Chem., 53, 1298-1302 (1981).
J. W. Jorgenson and K. D. Lukacs, Capillary Zone Electrophoresis, Science 222, 266-272 (Oct. 21, 1983).
P. Jandik et al., Electrophoretic Capillary Ion Analysis: Origins, Principles, and Applications, LC-GC 9(9), 634-645 (1991).
Dionex Corporation, Advertisement, LC-GC, 9(9) (1991).
H. Small, et al., Novel Ion Exchange Chromatographic Method Using Conductimetric Detection, Analytical Chemistry, 47(11), 1801-1809 (Sep. 1975).
D. L. Strong, et al., Electrodialytic Membrane Suppressor for Ion Chromatography, Anal. Chem., 61, 939-945 (1989).
D. L. Strong, et al., Electrodialytic Eluent Generation and Suppression, Journal of Chromatography 546, 159-173 (1991).
X. Huang, et al., On-Column Conductivity Detector for Capillary Zone Electrophoresis, Anal. Chem., 59, 2747-2749 (1987).
R. Wallingford, et al., Capillary Zone Electrophoresis with Electrochemical Detection, Anal. Chem., 59, 1762-1766 (1987).
M. C. Linharcs, et al., Use of an On-Column Fracture in Capillary Zone Electrophoresis for Sample Introduction, Anal. Chem., 63, 2076-2078 (1991).
C. R. Martin, et al., Dissolution of Perfluorinated Ion Containing Polymers, Anal. Chem., 54, 1639-1641 (1982).
Conductrometric Detection, Chapter 7, Ion Chromatography, Edited by Hamish Small, 149-189 (1989).
P. D. Dasgupta, Approaches to Ionic Chromatography, Chapter 6, Ion Chromatography, Edited by James G. Tarter, 191-367 (1987).
Dasgupta Purnendu K.
Li-Yuan Bao
Halldorson Burke M.
Niebling John
Starsiak Jr. John S.
Stevens Timothy S.
The Dow Chemical Company
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