Chemistry: electrical and wave energy – Apparatus – Electrophoretic or electro-osmotic apparatus
Patent
1994-05-26
1997-05-06
Schain, Howard E.
Chemistry: electrical and wave energy
Apparatus
Electrophoretic or electro-osmotic apparatus
204616, 204618, 204619, C25B 900
Patent
active
056267355
ABSTRACT:
Gradient gels are formed in a slab gel enclosure by introducing gel solution through a channel in one of the two or more spacers which set the thickness of the gel. The channel opens into the gel space through an aperture in the inner longitudinal edge of the spacer at approximately mid-length along the edge, and filling of the gel space with the solution is performed with the enclosure rotated onto its side. In certain embodiments of the invention, the opposing spacer contains an aperture as well, plus a channel leading from the aperture to the exterior of the enclosure, for venting of air from the gel space as the space is being filled with the gel-forming liquid.
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Anne-Lise Borresen, et al. Constant denaturant gel electrophoresis as a rapid screening technique for p53 mutations. Proc. Natl. Acad. Sci. USA. vol. 88, pp. 8405-8409,Oct. 1991 Medical Sciences.
Birgitte Smith-Sorensen, et al. Screening for mutations in human HPRT cDNA using the polymerase chain reaction (PCR) in combination with constant denaturant gel electrophoresis (CDGE). Mutation Research, 269 (1992) 41-53. 1992 Elsevier Science Publishers B.V.
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Bio-Rad Laboratories, Inc.
Delacroix-Muirheid C.
Schain Howard E.
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