Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...
Patent
1993-12-03
2000-08-22
Niebling, John
Chemistry: electrical and wave energy
Processes and products
Electrophoresis or electro-osmosis processes and electrolyte...
204606, 204612, 204616, 2504581, 2504591, 2504611, 356344, C25B 700, C25B 900
Patent
active
RE0368261
ABSTRACT:
An electrophoresis pattern reading system of fluorescent type is comprised of a detachable migration unit comprising a gel functioning as a base for electrophoresis and a gel-supporting body for supporting the gel; an electrophoresis unit, to which the migration unit is mounted, for performing electrophoresis by applying migrating voltage to the gel to which a sample labeled with a fluorescent substance is added; and a reading unit including an instrumentation subunit for reading an electrophoresis pattern, to which the migration unit is mounted after electrophoresis and which receives fluorescence emitted from the fluorescent substance of the sample on the gel upon application of light to the gel. For example, a plurality of the migration units and the electrophoresis units are provided, and each of the plural migration units are mounted to one reading unit in order after electrophoresis has been performed with the respective electrophoresis units for a long period of time, thereby reading the electrophoresis pattern in a short period of time.
REFERENCES:
patent: 3994593 (1976-11-01), Kato et al.
patent: 4087685 (1978-05-01), Froot
patent: 4130824 (1978-12-01), Amos et al.
patent: 4284897 (1981-08-01), Sawamura et al.
patent: 4354114 (1982-10-01), Karnaukhov et al.
patent: 4675095 (1987-06-01), Kambara et al.
patent: 4720788 (1988-01-01), Golias
patent: 4734578 (1988-03-01), Horikawa
patent: 4791310 (1988-12-01), Honig et al.
patent: 4810348 (1989-03-01), Sarrine et al.
patent: 4811218 (1989-03-01), Hunapiller et al.
patent: 4893008 (1990-01-01), Horikawa
patent: 5022757 (1991-06-01), Modell
patent: 5091652 (1992-02-01), Mathies et al.
patent: 5108179 (1992-04-01), Myers
patent: 5213673 (1993-05-01), Fujimiya et al.
patent: 5242567 (1993-09-01), Fujimiya et al.
European Patent Search, Apr. 15, 1996.
Peck et al. (1989) "Single Molecule fluorescence detection: Autocorrelation criterion and experimental realization with phycoerythrin," Proc. Natl. Acad. Sci. USA, 86, 4087-4091.
Mathies,R.A. and Stryer,L. (1986), Applications of Fluorescence in the Biomedical Sciences, Eds. Taylor,D.L., Waggonner,A.SA., Lanni,F., Murphy, R.F., and Birge,R. (Alan R. Liss, Inc., New York) pp. 129-140.
Nguyen,D.C.; Kellery, R.A.; Jett, J.H.; Martin, J.C. (1987), Detection of Single Molecules of Phycoerythrin in Hydrodynamically Focused Flows by Laser-Induced Fluorescence, Anal.Chem. 59, 2158-2161.
Peck, K; Stryer, L; Glazer, A.N.; Mathies, R.A. (1989) Single molecule fluorescence detection: Autocorrelation criterion and experimental realization with phycoertthrin Proc. Natl. Acad. Sci. USA, 86, 4087-4091.
Mathies,R.A.; Peck,K,; Stryer, L. (1990), Optimization of High-Sensitivity Fluorescence Detection, Anal.Chem. 62, pp. 1786-1791,
Glaser, A.N.; Peck, K.; Mathies, R.A. (1990), A stable double-stranded DNA-ethidium homodimer complex: Application to picogram fluorescence detection of DNA in agarose gels, Proc.Natl.Acad.Sci. USA, vol. 87, pp 3851-3855, May 1990 Biochemistry.
Ansorge, W.; Rosenthal, A.; Sproat, B.; Schwager, C.; Stegemann, J.; and Voss, H. (1988) Non-radioactive automated sequencing of oligonucleotides by chemical degradation;Nucleic Acids Research vol. 16, 2203-2206.
Smith, L.M.; Sanders, J.Z.; Kaiser, R.J.; Hughes, P.; Dodd, C.; Connell, C.R. Heiner, C.; Kent, S.B.H. & Hood, L.E. (1986), Fluorescence detection in automated DNA sequence analysis, Nature, vol. 321, 674-679.
Sanger, F.; Nicklen, S.; & Coulson, A.R. (1977), DNA Sequencing with chain-terminating inhibitors, Proc.Natl.Acad.Sci., vol. 74, No. 12, pp. 5463-5467.
Fujimiya Hitoshi
Koga Keigi
Nakajima Shigeo
Nasu Hisanori
Delacroix-Muirhead C.
Hitachi Software Engineering Co. Ltd.
Niebling John
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