Electrophoresis of nucleic acid fragments

Details Electrophoresis of nucleic acid fragments Electrophoresis of nucleic acid fragments

1992-04-03

1994-05-24

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, G01N 2726, G01N 27447

Patent

active

053145950

ABSTRACT:
A method for electrophoresis of nucleic acid fragments present in the solution which contains an amount, e.g., 0.2% or more, of a reagent, e.g., glycerol, dithiothreitol (DTT) and trehalose or other sugars, which interact to form a complex with borate or boric acid. The method includes applying the solution to an electrophoretic gel and electrophoresing those fragments into the gel in the presence of a buffer lacking boric acid, or a derivative thereof, which forms a chelate complex with the reagent and thereby causes distortion of electrophoresis of the fragments in a gel including such a buffer.

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