Electrophoresis method using vesicles

Details Electrophoresis method using vesicles Electrophoresis method using vesicles

1988-08-09

1991-04-09

Rosen, Sam

Chemistry: analytical and immunological testing

Involving diffusion or migration of antigen or antibody

Through a gel

435 4, 435 6, 435 711, 435 725, 435 75, 435 79, 435 14, 435 28, 436512, 436513, 436514, 436515, 436829, 436536, G01N 33561

Patent

active

050064738

ABSTRACT:
The electrophoresis method of the present invention employs a media which contains uniformly dispersed liposomes of phospholipid, or combinations of phospholipid and neutral lipid, which contain chromogenic materials or dye precursers. After electrophoresis of a test sample, the liposomes are lysed and the chromogen or dye material released. The chromogen or dye can be any signal producing substance including a chromogenic agent, and enzyme, a fluorogenic agent, or a chemiluminescent agent, but a detectable signal occurs only where the staining material is in close proximity to specific enzymes, effectors, analytes, or other color-inducing agents which have migrated through the gel during electrophoresis.

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Trampont et al-Chem. Abst. vol. 103 (1985) p. 11479j.

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