Electrophoresis imaging system

Details Electrophoresis imaging system Electrophoresis imaging system

1988-03-25

1990-06-05

Rosenberger, Richard A.

Optics: measuring and testing

By electrophoresis

356128353, G01N 2141, G01B 902

Patent

active

049308939

ABSTRACT:
A method and system for imaging unstained smaple material constituents on a gel electrophoresis unit. The system includes a gel electrophoresis unit, preferably having a pH gradient gel thereon, phase detection optics including a collimated light source, shearing interferometer, phase contrast microscope or schlieren microscope, an image digitizer, and data processing electronics, such as a computer, for determining the concentration of sample constituents at each position on the electrophoresis unit. The method relies on the difference in index of refraction between sample material and gel material to introduce optical path differences in light rays passing through the material. The modified light is subjected to shearing interferometry to locate gel-sample boundaries, phase imaging to directly image the sample, or a laser scanner for directly measuring the deviation in a light beam due to the sample.

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Advertisement for ATTO Schlierograph, model EDS-100, n.d.
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