Electrophoresis gels and gel holders having fiber spacers and me

Details Electrophoresis gels and gel holders having fiber spacers and me Electrophoresis gels and gel holders having fiber spacers and me

1995-12-12

1997-04-08

Gorgos, Kathryn

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

204619, 204620, G01N 2726, G01N 27447

Patent

active

056183984

ABSTRACT:
Gel holders for electrophoresis gels are made using clad fibers, particularly glass fibers as spacers between substrates. A plurality of fibers with an high-melting interior core and a low-melting external cladding are placed between a first planar substrate and a second planar substrate. The fibers are heated to a temperature sufficient to at least soften the exterior cladding of the fibers without softening the interior core of the fibers, and then cooled while they are in contact with the first and second substrates to resolidify the exterior cladding. This adheres the fibers to the first and second substrates, and forms a gel chamber between said first and second substrates. The gel chamber has a thickness defined by interior core of the fibers. The fibers may be heated before or after the second substrate is placed over the top of the fibers. The gel holders thus formed may be filled immediately with a gel forming solution such as a polyacrylamide, or they may be stored indefinitely and used as needed.

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Maniatis, Chapter 5, "Gel Electrophoresis". No Date Available.
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