Electrophoresis device and the use thereof

Details Electrophoresis device and the use thereof Electrophoresis device and the use thereof

2002-03-18

2008-12-02

Olsen, Kaj K (Department: 1795)

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

C204S455000, C204S459000, C204S601000, C204S605000, C204S610000

Reexamination Certificate

active

07459069

ABSTRACT:
The invention relates to a device for electrophoretically separating molecules. The inventive device comprises: at least one plate having a top side and an underside; a first separating channel provided for receiving at least one first separating medium; at least one second channel, which is orthogonal to said first separating channel and which is provided for receiving a second separating medium; optionally comprises implements provided for filling the device with reagents, solvents, buffers, separating media and/or for loading the device with a sample to be separated, and; terminals for applying electric separating voltage to the separating channels. In the device, the first separating channel is located on the plate top side and comprises at least one first opening that leads to the plate underside, whereby the second separating channel is located on the plate underside and comprises at least one second opening. The first opening of the first channel and the second opening of the second channel are connected to one another.

REFERENCES:
patent: 4101401 (1978-07-01), Hoefer
patent: 4385974 (1983-05-01), Shevitz
patent: 5599432 (1997-02-01), Manz et al.
patent: 6013165 (2000-01-01), Wiktorowicz et al.
patent: 6284113 (2001-09-01), Bjornson et al.
patent: 6607644 (2003-08-01), Apffel, Jr.
patent: 198 26 020 (1999-12-01), None
patent: 0 544 969 (1993-06-01), None
patent: 0 977 030 (2000-02-01), None
H. Becker et al., Planar quartz chips with submicron channels for two-dimensional capillary electrophoresis applications, Journal of Micromech. & Microeng., New York, NY, vol. 8, No. 1, Mar. 1, 1998, pp. 24-28, XP-002088541.
A. Gorg et al, The current state of two-dimensional electrophoresis with immobilized pH gradients, Electrophoresis 2000, Weinheim, Germany, vol. 21, No. 6, Apr. 2000, pp. 1037-1053, XP-001015374.
“High Resolution Two-Dimensional Electrophoresis of Proteins”, Patrick H. O'Farrell, The Journal of Biological Chemistry, vol. 250, No. 10, Issue of May 25, pp. 4007-4021, 1975.
“Adaptation of Capillary Isoelectric Focusing to Microchannels on a Glass Chip”, Oliver Hofmann, Diping Che, Kenneth A. Cruickshank, And Uwe R. Müller, Anal. Chem. 1999, 71, 678-686.
“Room-Temperature Imprinting Method for Plastic Microchannel Fabrication”, Jingdong Xu, Laurie Locascio, Michael Gaitan, and Cheng S. Lee, Anal. Chem. 2000, 72, 1930-1933.
“SDS Capillary Gel Electrophoresis of Proteins in Microfabricated Channels”, Shao Yao, Deon S. Anex, W. Brett Caldwell, Don W. Arnold, Katherine B. Smith, and Peter G. Schultz, Proc. Natl. Acad. Sci. USA, vol. 96, pp. 5372-5377, May 1999 Chemistry.

Affiliated with

Also associated with

Rating

Electrophoresis device and the use thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Electrophoresis device and the use thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Electrophoresis device and the use thereof will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4020603