Electrophoresis chip

Chemistry: electrical and wave energy – Apparatus – Electrophoretic or electro-osmotic apparatus

Reexamination Certificate

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Details

C204S601000

Reexamination Certificate

active

07879215

ABSTRACT:
An electrophoresis chip is provided with a channel that is filled with a solution in which a sample is dissolved, the electrophoresis chip being configured to carry out electrophoresis by applying voltage along the channel in a state in which the channel is hermetically sealed to separate the sample in the channel, and after this electrophoresis is performed, to carry out mass spectrometry by scanning a laser along the channel in a state in which the channel is open to an environment in which gas is present. A pattern is formed on the bottom surface of the channel to hold the solution as droplets. This pattern is made into a pattern in which hydrophilic areas are surrounded by hydrophobic areas and side-walls of the channel.

REFERENCES:
patent: 7586091 (2009-09-01), Takahashi et al.
patent: 2002/0023840 (2002-02-01), Johnson et al.
patent: 2008/0023331 (2008-01-01), Hattori
patent: 2006266795 (2006-10-01), None
patent: 03/071263 (2002-12-01), None
patent: 2005124332 (2005-12-01), None
Ken Tseng et al., “Fabrication and Design of Open Microchannels for Capillary Electrophoresis Separations and Matrix-assisted Laser/Desorption Mass Spectrometry Analysis”, SPIE Conference on Micro-and Nanofabricated Structures and Devices for Biomedical Environmental Application II, San Jose, California, (Jan. 1999), SPIE vol. 3606, pp. 137-148.
Jun Liu et al., “Electrophoresis Separation in Open Microchannels. A Method for Coupling Electrophoresis and MALDI=MS”, Analytical Chemistry, vol. 73, No. 9, (May 1, 2001), pp. 2147-2151.
Michelle L.-S Mok et al., “Capillary Isoelectric Focusing in Pseudo-closed Channel Coupled to Matrix Assisted Laser Desorption/Ionization Mass Spectrometry for Protein Analysis”, The Analyst, 2004, vol. 129, pp. 109-111.

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