Electrophoresis cassette system with apparatus and method for fi

Chemistry: electrical and wave energy – Apparatus – Coating – forming or etching by sputtering

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2041828, G01N 2726

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active

049293290

ABSTRACT:
An electrophoresis cassette system which when used with a detector can automatically perform an analysis of radio-labelled molecules. The cassette system is easily assembled and may be reused. The cassette includes a new type of side spacer which incorporates nylon monofilament and which includes a buffer zone for increased accuracy. A new clamp and other improvements further enhance the accuracy of the cassette and reduce variations in the electric field at the bottom and sides of the cell to provide increased accuracy. A new cassette filling system is shown for use with the cassette which permits rapid filling of a cassette with gel material while providing a more uniform gel layer than was previously attainable.

REFERENCES:
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patent: 4339327 (1982-07-01), Tyler
patent: 4417967 (1983-11-01), Ledley
patent: 4524769 (1986-11-01), Simada et al.
patent: 4576693 (1986-03-01), Kreisher et al.
patent: 4762743 (1988-08-01), von Alven et al.
A. C. Arcus; "Vertical Gel Electrophoretic Analysis in Slabs: A Simple Apparatus" Laboratory Practice, Apr. 1976.
Instruction Manual for "Poker Face" Sequencer, SE 1500, Hoffer Scientic Instruments, 1985.
Gel Electrophoresis of Nucleic Acids; Edited by D. Rickwood and B. D. Hanes; pp. 39-41, 81-85, 88; I. R. L. Press; Oxford, England; 1982.
Bethesda Research Laboratories Brochure Entitled "Priceless Performance, Affordable Price" 1984.

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