Electrophoresis-based sequencing of oligosaccharides

Details Electrophoresis-based sequencing of oligosaccharides Electrophoresis-based sequencing of oligosaccharides

1990-07-27

1994-02-08

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

435 18, 435212, 436 94, 536127, 536 185, G01N 27447, G01N 2726, G01N 3348, C12Q 134

Patent

active

052845583

ABSTRACT:
The electrophoretic isolation and monosaccharide sequence determination of a neutral or weakly acidic oligosaccharide species of interest are disclosed. A labeling compound and a charged group are coupled to the reducing end of the species of interest, thereby facilitating electrophoretic separation and detection of the separated species. The resolved species of interest can then be recovered from the electrophoretic medium, for example, by electrophoretic transfer to a charged solid support.
Following isolation, monosaccharide units can be cleaved successively from the non-reducing end of the species of interest to reveal the monosaccharide sequence. The identity of each monosaccharide unit is determined by correlating cleavage data with known exoglycosidase specificites.

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