Electrophoresis apparatus and method

Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...

Reexamination Certificate

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C204S644000

Reexamination Certificate

active

06923896

ABSTRACT:
An electrophoretic apparatus comprising: a first electrolyte chamber containing a first electrode; a second electrolyte chamber containing a second electrode; a first sample chamber disposed between the first and second electrolyte chambers and proximate to the first electrolyte chamber; a second sample chamber disposed between the first sample chamber and the second electrolyte; three ion-permeable barriers separating the first electrolyte chamber, the first sample chamber, the second sample chamber, and the second electrolyte chamber, respectively, wherein the ion-permeable barriers impede convective mixing of the contents in each of the respective chambers; a first electrolyte reservoir and a second electrolyte reservoir in fluid communication with the first and second electrolyte chambers, respectively; a first sample reservoir and a second sample reservoir in fluid communication with the first and second sample chambers, respectively; means adapted for communicating a first electrolyte and a second electrolyte between the respective electrolyte chambers and reservoirs; means adapted for communicating a first fluid and a second fluid between the respective sample chambers and reservoirs, wherein at least one of the first and second fluid contains at least a sample, wherein application of an electric potential causes migration of at least one component through at least one of the ion-permeable barriers.

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