Electrophoresis and vacuum molecular transfer apparatus

Details Electrophoresis and vacuum molecular transfer apparatus Electrophoresis and vacuum molecular transfer apparatus

1991-04-30

1993-06-08

Niebling, John

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

2041828, 2041801, G01N 2726, G01N 27447

Patent

active

052175928

ABSTRACT:
An apparatus which includes the combination of a submarine gel tank, for the electrophoresic separation of, for example, nucleic acid molecules on agarose slab gels, with a vacuum applying means which transfers the separated molecules from the gel, without further handling, to a filter membrane by means of a controlled vacuum.

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Bethesda Research Laboratories, Inc., "Catalogue & Reference Guide", Aug. 1983, p. 51.
M. Peferoen et al. "Vacuum-blotting: a new simple and efficient transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to nitrocellulose" FEBS Letters vol. 145, No. 2 (Aug. 1982) pp. 369-372.
E. M. Southern, "Detection of Specific Sequences Among DNA Fragments Separated by Gel Electrophoresis", Journal of Molecular Biology, (1975), 98, 503-517.
M. Bittner et al., "Electrophoretic Transfer of Proteins and Nucleic Acids from Slab Gels to Diazobenzyloxymethyl Cellulose or Nitrocellulose Sheets", Analytical Biochemistry 102, 459-471 (1980).

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