Electrophoresis and transfer for

Details Electrophoresis and transfer for Electrophoresis and transfer for

1988-10-19

1989-12-26

Niebling, John F.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, 204301, 2041821, G01N 2728, G01N 2726

Patent

active

048896063

ABSTRACT:
A method and apparatus for use in biological testing. The apparatus comprises electrophoresis apparatus comprising a tank (1) the interior of which is formed with a raised portion (5) which splits the tank into the two compartments (6,7) containing electrophoresis electrolyte. Electrodes (8) are provided in each compartment. Electrophoresis is carried out through a gel (not shown) supported by a rigid porous plate (11). The gel is cast onto the porous plate with a transfer membrane (not shown) between the porous plate and the gel. Further electrodes (20,21) are mounted below and above the gel respectively. The method of the invention comprises a first stage using the electric field produced by the electrodes (8) to draw the samples through the gel and a second stage using the electric field produced by the electrodes (20,21) to draw the distributed macromolecules onto the transfer membrane.

REFERENCES:
patent: 4589965 (1986-05-01), Kreisher
patent: 4622124 (1986-11-01), Kreisher et al.
patent: 4726889 (1988-02-01), Love et al.
Dietzen, G. et al., "A Reliable Method for Recovery of DNA Fragments for Agarose and Acrylamide Gels" Analytical Biochemistry 112 (1981), pp. 295-298.

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