Chemistry: electrical and wave energy – Apparatus – Electrophoretic or electro-osmotic apparatus
Patent
1995-10-19
1996-07-23
Niebling, John
Chemistry: electrical and wave energy
Apparatus
Electrophoretic or electro-osmotic apparatus
204461, 204456, 204608, 204616, 356344, G01N 2726, G01N 27447
Patent
active
055386130
ABSTRACT:
An apparatus for detecting fluorescently-labeled molecules moving in an electrophoretic separation medium. The apparatus utilizes a scanning detection system in which multiple fluorophores are efficiently and simultaneously detected using dichroic mirrors to allow simultaneous detection of fluorescently-labeled molecules in ultrathin gels labeled with several fluorophores and to permit operation at speeds ten times faster than prior art gel separations. The apparatus also utilizes a detection system where lightweight collection optics are in motion while detection optics are fixed in a remote location, thereby allowing exceptionally high-speed scanning.
REFERENCES:
patent: 4284412 (1981-08-01), Hansen et al.
patent: 4729947 (1988-03-01), Middendorf et al.
patent: 4811218 (1989-03-01), Hunkapiller et al.
patent: 4870004 (1989-09-01), Conroy et al.
patent: 4881812 (1989-11-01), Ohkubo et al.
patent: 4885696 (1989-12-01), Hara
patent: 4941092 (1990-07-01), Hara et al.
patent: 4971671 (1990-11-01), Slater et al.
patent: 5051162 (1991-09-01), Kambara et al.
patent: 5062942 (1991-11-01), Kambara et al.
patent: 5069769 (1991-12-01), Fujimiya et al.
patent: 5091652 (1992-02-01), Mathies et al.
patent: 5100529 (1992-03-01), Fujii
patent: 5171534 (1992-12-01), Smith et al.
Brumley and Smith, 1990, Nucleic Acids Research, 19:4121-4126.
Chen, E. and Seeburg, P., 1985, "Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNA," D.N.A. 4:165.
Maxam, A. and Gilbert W., 1970, "A New Method for Sequencing DNA," Proc. Natl. Acad. Sci. U.S.A., 74:560.
Sanger F., et al., 1977, "DNA Sequencing with Chain-Terminating Inhibitors," Proc. Natl. Acad. Sci. U.S.A., 74:5463.
Smith, et al., 1986, Nature, 321:674-679.
Brumley Robert L.
Luckey John A.
GeneSys Technologies, Inc.
Niebling John
Starsiak Jr. John S.
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