Electronically mediated nucleic acid amplification in NASBA

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S006120, C435S091200, C435S283100, C435S285100, C435S287200, C536S022100, C536S023100, C536S025320, C536S024300, C536S024310, C536S024320

Reexamination Certificate

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07070961

ABSTRACT:
A method of improving amplification of nucleic acids using a nucleic acid sequence-based amplification (“NASBA”) method is provided wherein target nucleic acids and NASBA primers are electronically addressed to electronically addressable capture sites of a microchip. This improvement uses electronically induced hybridization of the target nucleic acids to the primers. The primers may be solution-based or immobilized on the capture sites of the microchip.

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