Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing
Reexamination Certificate
2001-08-27
2004-07-27
Redding, David A. (Department: 1744)
Chemistry: molecular biology and microbiology
Apparatus
Including measuring or testing
C435S288500, C435S288600, C435S288700, C204S403130, C204S603000
Reexamination Certificate
active
06767731
ABSTRACT:
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to the fields of molecular biology and nucleic acid analysis. In particular, the invention relates to methods, composition and apparatus for electron-induced fluorescent DNA sequencing.
2. Background
With the advent of the human genome project, a need developed for improved methods of sequencing nucleic acids such as DNA and RNA. Genetic information is stored in the form of very long molecules of deoxyribonucleic acid (DNA), organized into chromosomes. The twenty-three pairs of chromosomes in the human genome contain approximately three billion bases of DNA sequence. This DNA sequence information determines multiple characteristics of each individual, such as height, eye color and ethnicity. Many common diseases, such as cancer, cystic fibrosis, sickle cell anemia and muscular dystrophy are based at least in part on variations in DNA sequence.
Determination of the entire sequence of the human genome has provided a foundation for identifying the genetic basis of such diseases. However, a great deal of work remains to be done to identify the genetic variations associated with each disease. This requires DNA sequencing of portions of chromosomes in individuals or families exhibiting each such disease, in order to identify specific changes in DNA sequence that promote the disease. Ribonucleic acid (RNA), an intermediary molecule required for processing of genetic information, can also be sequenced to identify the genetic basis of various diseases.
Existing methods for nucleic acid sequencing, based on detection of fluorescently labeled nucleic acids that have been separated by size, are limited by the length of the nucleic acid that can be sequenced. Typically, only 500 to 1,000 bases of nucleic acid sequence can be determined at one time. This is much shorter than the length of the functional unit of DNA, referred to as a gene, which can be tens or even hundreds of thousands of bases in length. Determination of a complete gene sequence requires that many copies of the gene be produced, cut into overlapping fragments and sequenced, after which the overlapping DNA sequences can be assembled into the complete gene. This process is laborious, expensive, inefficient and time-consuming.
REFERENCES:
patent: 5079169 (1992-01-01), Chu et al.
patent: 5202231 (1993-04-01), Drmanac et al.
patent: 5332666 (1994-07-01), Prober et al.
patent: 5436130 (1995-07-01), Mathies et al.
patent: 5776674 (1998-07-01), Ulmer
patent: 5821060 (1998-10-01), Arlinghaus et al.
patent: 5846727 (1998-12-01), Soper et al.
patent: 5972619 (1999-10-01), Drmanac et al.
patent: 6018041 (2000-01-01), Drmanac et al.
patent: 6025136 (2000-02-01), Drmanac
patent: 6045996 (2000-04-01), Cronin et al.
patent: 6063573 (2000-05-01), Kayyem
patent: 6083695 (2000-07-01), Hardin et al.
patent: 6136543 (2000-10-01), Anazawa et al.
patent: 6188167 (2001-02-01), Endo et al.
patent: 6225068 (2001-05-01), Wolfrum
patent: 6232062 (2001-05-01), Kayyem et al.
patent: 6238871 (2001-05-01), Köster
patent: 6447724 (2002-09-01), Jensen et al.
patent: WO 99/57321 (1999-11-01), None
patent: WO 00/36152 (2000-06-01), None
patent: WO 01/18246 (2001-03-01), None
patent: WO 01/34302 (2001-05-01), None
Burns, Mark A. et al., “Microfabricated structures for integrated DNA analysis”,Proc. Natl. Acad. Sci., 93:5556-5561, May 1996.
Knight, James B. et al., “Hydrodynamic Focusing on a Silicon Chip: Mixing Nanoliters in Microseconds”,Physical Review Letters, 80(17):3863-3866, Apr. 1998.
Machara, Nicholas P. et al., “Efficient detection of single molecules eluting off an optically trapped microsphere”,Bioimaging, 6:33-42, 1998.
Chou, Hou-Pu et al., “A Microfabricated device for sizing and sorting DNA molecules”,Proc. Natl. Acad. Sci., 96:11-13, 1999.
Chen, Liaohai et al., “Highly sensitive biological and chemical sensors based on reversible fluorescence quenching in a conjugated polymer”,PNAS, 96(22):12287-12292, Oct. 1999.
Meller, Amit et al., “Rapid nanopore discrimination between single polynucleotide molecules”,PNAS, 97(3):1079-1084, Feb. 2000.
Rusu, Cristina et al., “Fabrication of micromachined pipettes in a flow channel for single molecule handling of DNA”,Proceedings MEMS, Japan, 2000.
Schafer, Burk et al., “Single molecule DNA restriction analysis in the light microscope”,Single Molecules, 1:33-40, 2000.
Chen, Liaohai et al., “Tuning the properties of conjugated polyelectrolytes through surfactant complexation”,Journal American Chemical Society, Apr. 2000.
Baumann, Christoph G. et al., “Stretching of single collapsed DNA molecules”,Biophysical Journal, 78:1965-1978, Apr. 2000.
Hegner, Martin, “DNA handles for single molecule experiments”,Single Molecules, 1(2):139-144, 2000.
Huser, Thomas et al., “Single chain spectroscopy of conformational dependence of conjugated polymer photophysics”,PNAS, 97(21)11187-11191, Oct. 2000.
Tretiak, S. et al., “CEO/semiempirical calculations of UV-visible spectra in conjugated molecules”,Chemical Physics Letters, 331:561-568, Dec. 2000.
Intel Corporation
Redding David A.
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