Electromicroinjection of particles into living cells

Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge

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204299R, 604 21, 12830318, 128784, 128786, 435296, 4351721, 4351722, 4351723, B01D 5702, A61N 130, A61B 1736, C12N 1500

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047847379

ABSTRACT:
Method and apparatus for introducing particles into living cells. Fluorescently-stained human chromosomes are introduced into cultured, mitotic Chinese hamster cells using electromicroinjection. The recipient cells frequently survived the physiological perturbation imposed by a successful chromosome injection. Successfully injected recipient cells maintained viability as evidenced by their ability to be expanded.

REFERENCES:
Elaine G. Diacumakos et al., "A Microsurgical Methology for Human Cells in Vitro: Evolution and Applications," 65 Proc. Nat. Acad. Sci., (No. 4), 911 (1970).
Elaine G. Diacumakos, "Methods for Micromanipulation of Human Somatic Cells in Culture,", in Methods in Cell Biology, (Academic Press, Inc., New York, 1973), vol. VII, Chap. 17, p. 287.
Munehiko Ocho et al., "Microinjection of Nucleic Acids into Cultured Mammalian Cells by Electrophoresis", 35 Acta. Med. Okayama, (5), 381 (1981).
F. Andrew Ray, "The Introduction of Morphologically Intact Human Chromosomes Into Chinese Hamster Mitotic Cells by Electromicroinjection", thesis submitted in partial fulfillment of requirements for the degree of Master of Science in Medical Sciences.

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