Electrolytic analytical methods

Details Electrolytic analytical methods Electrolytic analytical methods

1995-06-05

1996-08-13

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 14, 435 25, 435 26, 435 28, 435183, 435190, 435192, 436 63, 436 71, 424450, C12Q 100, G01N 3392, C12N 900

Patent

active

055455190

ABSTRACT:
Enzyme electrodes having the enzyme enclosed within liposomes supported on, or in a membrane and immobilized therein are provided. The liposomes may be made using lipids and conveniently by "reverse phase evaporation". The enzyme electrodes incorporate conventional electrode elements, e.g., THE Clark electrode pair (Platinum/Silver) and additional membranes or screens, and used particularly in amperometric measurements. The enzyme can be an oxidase and is useful for measurement, directly or indirectly, of low molecular weight species generated by enzymic action, and effective for study of biological fluids.

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Orbit Search Service, File Analytical Abstracts, Accession No. 52-04D00171, Anal. Lett., 22(5): Apr. 1989, Wu, T.-G. et al, "Potentiometric enzyme-amplified flow-injection analysis detectin system: behaviour of free and liposome-released peroxidase", pp. 1107-1124.

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