Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1985-06-24
1987-05-05
Warren, Charles F.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435 1, 435 2, 435241, 435260, 424127, 424128, 424153, 424154, 514 23, 514 2, C12N 500, A01N 102
Patent
active
046632891
ABSTRACT:
Processes and compositions are provided for in vitro use in tissue culture media (preferred), perfusion media, and incubation media. Thus, balanced salt solutions are provided wherein the milliequivalent ratio of sodium cations to chloride anions is normalized and wherein optionally at least one near-equilibrium couple is incorporated (bicarbonate.sup.- /carbon dioxide, L-lactate.sup.- /pyruvate.sup.-, and d-betahydroxybutyrate.sup.- /acetoacetate.sup.-. Regulation of intracellular and extracellular pH, and normalization of intracellular redox state and phosphorylation state is achievable.
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Moskowitz M.
Warren Charles F.
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