Electroenzymatic reactor and method for enzymatic catalysis

Chemistry: electrical and wave energy – Apparatus – Electrolytic

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204252, 204275, C25B 900, C25C 700, C25D 1700

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061267953

ABSTRACT:
An electroenzymatic reactor comprises a reaction vessel having a working electrode compartment. Within the working electrode compartment is a reactor solution containing a redox enzyme, a redox carrier, and an oxidizable or reducible substrate. Also within the working electrode compartment is a reference electrode and a metal oxide working electrode capable of being held close to the redox potential of the redox carrier. A counter electrode is provided outside the working electrode compartment. Necessary molecular oxygen or hydrogen is supplied to the reaction by water electrolysis at the counter electrode. Hydrogen peroxide that is generated at the working electrode or counter electrode is decomposed by a screen situated near the hydrogen-peroxide generating electrode.

REFERENCES:
patent: 3919052 (1975-11-01), Fresnel et al.
patent: 4318784 (1982-03-01), Higgins et al.
patent: 4655885 (1987-04-01), Hill et al.
patent: 4761208 (1988-08-01), Gram et al.
patent: 5403450 (1995-04-01), Mellor et al.
patent: 5510512 (1996-04-01), Strutz
Albertson et al., "Spectroelectrochemical Determination of Heterogeneous ctron Transfer Rate Constants," Analytical Chemistry, vol. 51, No. 4, Apr. 1979, pp. 556-560.
Allen et al., "Surface Modifiers for the Promotion of Direct Electrochemistry of Cytochrome c," J. Electroanal. Chem., 178 (1984), pp. 69-86 no month available.
Armstrong, "Probing Metalloproteins by Voltammetry," Structure and Bonding, vol. 72, 1990, pp. 137-221 no month available.
Brewer et al., "Single Turnover Kinetics of the Reaction between Oxycytochrome P-450.sub.cam and Reduced Putidaredoxin," The Journal of Biological Chemistry, vol. 263, No. 2, Jan. 1988, pp. 791-798.
Brewer et al., "Single Turnover Studies with Oxy-cytochrome P-450.sub.cam," Archives of Biochemistry and Biophysics, vol. 249, No. 2, Sep. 1986, pp. 515-521.
Eble et al., "NADH- and Oxygen-Dependent Multiple Turnovers of Cytochrome P-450-CAM without Putidaredoxin and Putidaredoxin Reductase," Biochemistry 1984, vol. 23, No. 9, pp. 2068-2073 no month available.
Elliott et al., "Electrochemical Properties of Polycrystalline Tin Oxide," J. Electrochem. Soc.: Electrochemical Science, Nov. 1970, pp 1343-1348.
Estabrook et al., "Application of Electrochemistry for P450-Catalyzed Reactions," Methods in Enzymology, vol. 272, 1996, pp. 44-51 no month available.
Faulkner et al., "Electrocatalytically Driven .omega.-hydroxylation of fatty acids using cytochrome P450 4A1," Proc. Natl. Acad. Sci. USA, vol. 92, Aug. 1995, pp. 7705-7709.
Fruetel et al., "Calculated and Experimental Absolute Stereochemistry of the Styrene and .beta.-Methylstyrene Epoxides Formed by Cytochrome P450.sub.cam," Journal American Chemical Society, vol. 114, No. 18, 1992, pp. 6987-6993 no month available.
Grayson et al., "Tetralin as a Substrate for Camphor (Cytochrome P450) 5-Monooxygenase," Archives of Biochemistry and Biophysics, vol. 332, No. 2, Aug. 1996, pp. 239-247, Article No. 0338.
Gunsalus et al., "Cytochrome P-450.sub.cam Substrate and Effector Interactions," Annals New York Academy of Sciences, 107 (1973), pp. 107-117 no month available.
Ishimura, Yuzura, "Oxygen Activation and Transfer," Cytochrome P-450, Second Edition, pp. 80-91, 186-205 (1993). no month available.
Kazlauskaite et al., "Direct electrochemistry of cytochrome P450cam," Chem. Commun., 1996, pp. 2189-2190 no month available.
Lipscomb et al., "Autooxidation and Hydroxylation Reactions of Oxygenated Cytochrome P-450cam," The Journal of Biological Chemistry, vol. 251, No. 4, Feb. 1976, pp. 1116-1124.
Mohr, et al., "Aspects of Application of Cytochrome P-450 and Related Systems in Substrate Hydroxylation," Abstr., Pharmazie, Jul. 1978, vol. 33, No. 7, pp. 415-418.
Mohr, et al.; "Hemoproteins and Related Models in Hydroxylation Reactions of Organic Compounds," Abstr., J. Mol. Catal., 1981, vol. 13, No. 2, pp. 147-163 no month available.
Mohr, et al., "Use of Cytochrome P-450 and Iron Porphyrin as Catalysts in Hydroxylation Reactions in Combination With Electrochemical Systems," Abstr., Appl. Biochem. Microbiol., vol. 18, No. 4, 1982, pp. 377-384 no month available.
Mueller, Ernest J. , Paul J. Loida, and Stephen G. Sligar, "Twenty-five Years of P450cam Research," Cytochrome P450, Structure, Mechanism, and Biochemistry, Second Edition, 1995, Chapter 3, pp. 83-124. no month available.
Nishiyama et al., "Aminosilane modified indium oxide electrodes for direct electron transfer of ferredoxin," Journal of Electroanalytical Chemistry, vol. 373, (1994), pp. 255-258 no month available.
Pederson et al., "Redox and Ligand Dynamics in P450cam-Putidaredoxin Complexes," Microsomes and Drug Oxidations, Proceedings of the Third International Symposium Berlin, Jul. 1976, pp. 274-283.
Pugh, "Immobilized Redox Enzymes and Their Use as Catalysts for Fine Chemical Synthesis," Abstr., NATO ASI Ser., Ser. C, 1986, 178 (Enzymes Catal. Org. Synth.), pp. 217-232 no month available.
Pochapsky et al., "An NMR-Derived Model for the Solution Structure of Oxidized Putidaredoxin, a 2-Fe, 2-S Ferredoxin from Pseudomonas," Biochemistry, vol. 33, No. 21, 1994, pp. 6424-6432 no month available.
Reipa et al., "Surface-enhanced Raman spectroscopy (SERS) evidence of charge transfer between putidaredoxin and a silver electrode," Journal of Electroanalytical Chemistry, 395, Jan. 1995, pp. 299-303.
Reipa et al., "A direct electrode-driven P450 cycle for biocatalysis," Proc. Natl. Acad. Sci. USA, vol. 94, Dec. 1997, pp. 13554-13558.
Reipa et al., "Spectroscopic Real-Time Ellipsometry of Putidaredoxin Adsorption on Gold Electrodes," Abstr., Langmuir, 1997, vol. 13, No. 13, pp. 3508-3514 no month available.
Ryan et al., "Some Considerations in Spectroelectrochemical Evaluation of Homogeneous Electron Transfer Involving Biological Molecules," Analytical Chemistry, vol. 47, No. 6, May 1975, pp. 885-890.
Scheller, et al., "Electrochemical Investigations on the Oxygen Activation by Cytochrome P-450," Abstr., Acta. Biol. Med. Ger., vol. 38, No. 2-3, 1979, pp. 503-510 no month available.
Schubert, et al., "Application of Cytochrome P-450 in Enzyme Reactors and Enzyme Electrodes," Abstr., Pharmazie, 1985, vol. 40, No. 4, pp. 235-239 no month available.
Sligar et al., "Chemical mechanisms for cytochrome P-450 hydroxylation: Evidence for acvlation of heme-bound dioxygen," Proc. Natl Acad. Sci USA, vol. 77, No. 3, Mar. 1980, pp. 1240-1244.
Sligar et al., "A thermodynamic model of regulation: Modulation of redox equilibria in camphor monoxygenase," Proc. Nat. Acad. Sci USA, vol. 73, No. 4, Apr. 1976, pp. 1078-1082.
White et al., "Regioselectivity in the Cytochromes P-450: Control by Protein Constraints and by Chemical Reactivities," Archives of Biochemistry and Biophysics, vol. 228, No. 2, Feb., 1984, pp. 493-502.
Wong et al., "Characterization of Mercaptoethylamine-Modified Gold Electrode Surface and Analyses of Direct Electron Transfer to Putidaredoxin," American Chemical Society, Dec. 1995, pp. 4818-4822.
Wong, et al., "Development of a Novel Bioconversion Process Based on a P450cam Subsystem Driven by an Electrode Instead of NADH," Abstr. Pap. Am. Chem. Soc., 1996, 211 Meet., Pt. 1 no month available.
Yildirim, "Autooxidation and Hydroxylation Reactions of Oxygenated Cytochrome P-450cam," University of California, 1991, pp. 1-16 no month available.
Zhang et al., "Direct electron injection from electrodes to cytochrome P450 .sub.cam III biomembrane-like films," J. Chem. Soc., Faraday Trans., 1977, pp. 1769-1774 no month available.

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