Electroelution of oligonucleotides from gel matrices

Details Electroelution of oligonucleotides from gel matrices Electroelution of oligonucleotides from gel matrices

2011-06-21

2011-06-21

Noguerola, Alex (Department: 1759)

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

C204S613000

Reexamination Certificate

active

07964074

ABSTRACT:
The invention can be used to purify and extract a target oligonucleotide from a gel substrate. The current invention extracts the target oligonucleotide so quickly that burn-off against the positive electrode is greatly reduced, yielding high optical density and purity. The need for an osmotic membrane or heavy salt/light salt barrier to capture the oligonucleotide is eliminated. The invention does not require a high salt concentration and therefore does not require a desalting column that is time-consuming and reduces yield.

REFERENCES:
patent: 4747918 (1988-05-01), Wassenberg, II
patent: 2001/0032786 (2001-10-01), Anderson et al.
patent: 63-15148 (1988-01-01), None
JPO English language abstract of Ito JP 63015148 A patent published Jan. 22, 1988.
English language translation of Itoh et al. JP 63-015148 A, translated Oct. 2010.

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