Electroelution of macromolecules

Details Electroelution of macromolecules Electroelution of macromolecules

1987-10-22

1989-08-22

Niebling, John F.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

G01N 2728

Patent

active

048593028

ABSTRACT:
Macromolecules are electroeluted from a matrix by placing the matrix containing the macromolecules of interest in a container, forcing the matrix toward the tip of the container, e.g., by centrifugation, providing an opening at the tip of the container large enough to pass the component, but not the matrix, inserting that container inside another container, placing an electrode in each container together with enough electrophoresis buffer to immerse each electrode, and applying a voltage across the electrodes for a time and under conditions sufficient to cause the macromolecules but not the matrix to migrate from the first container into the second. A kit for performing the electroelution is also described.

REFERENCES:
patent: 4062731 (1977-12-01), Snoke
patent: 4576702 (1986-03-01), Peck et al.
patent: 4576703 (1986-03-01), Peck
patent: 4608147 (1986-08-01), Clad
patent: 4747918 (1988-05-01), Wassenberg
Ahokas, H., Nucleic Acids Research, vol. 15, No. 16, p. 6759 (1987).

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