Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1986-06-18
1988-05-31
Niebling, John F.
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
204299R, 436516, 436515, G01N 2728, G01N 2730
Patent
active
047479183
ABSTRACT:
An electroelution apparatus having at least one container for receiving a liquid, wherein the liquid volume of the container is less than 50 ml, a first electrode in the container on one side of the container and a second electrode in the container on the other side of the container, means for applying a sufficient voltage to the first and second electrodes to electrophoretically elute a charged biological molecule from a gel placed between the first and second electrodes, and means for interrupting the application of the voltage to the first and second electrodes. The elution time is 10 minutes or less, and the ratio of the volume of the gel to the volume of the liquid is greater than 1:100. Also disclosed is a method for eluting a charged biological molecule from a gel, comprising the steps of placing the gel in the container of the disclosed apparatus, substantially immersing at least a portion of the gel containing the biological molecule in a liquid, wherein the total volume of liquid is less than 50 ml, applying a voltage to the electrodes for a time of 10 minutes or less, and eluting the biological molecule.
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Bellomy William
Hsing Ben C.
Niebling John F.
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