Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-04-15
2000-12-26
Housel, James C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 5, 435 6, 435 71, 435 8, 435808, 435968, 435975, 422 52, 422 90, 422 98, 436501, 436546, 436800, 436805, 436806, 436807, 436 84, 436 72, 935 78, C12Q 170, G01N 3353, G01N 2176, G01N 33566
Patent
active
061657298
ABSTRACT:
Electrochemiluminescent assay methods for determining an analyte of interest, which methods include forming one or more compositions which include, in the aggregate, (a) a sample to be tested for the analyte of interest, (b) a component capable of specifically binding with the analyte, (c) a label reagent which, when oxidized, is capable of electrochemiluminescence, (d) an amine which, when oxidized, forms a reducing agent, and (e) an electrolyte solution; subjecting such a composition containing the sample to conditions sufficient for specific binding to occur between the analyte of interest, if present, and one or more of the other components in the composition; thereafter, a composition containing the label reagent and amine, and reflecting the outcome of applying the aforementioned binding conditions, is exposed to conditions, such that both the label reagent and the amine are oxidized, the amine forms a reducing agent which interacts with the label reagent, electrochemiluminescence occurs, and the luminescence emitted from the label reagent is used to determine the analyte of interest.
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Leland Jonathan Kent
Powell Michael Joseph
Gabel Gailene R.
Housel James C.
Hyperion Catalysis International Inc.
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