Electrochemiluminescent assay

Details Electrochemiluminescent assay Electrochemiluminescent assay

1996-02-05

1998-09-08

Leary, Louise N.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435975, 435 23, 435 19, 435817, 435968, 205255, 205257, 205264, 420461, 420462, C12Q 134, C12Q 137, G01N 3353, C12N 144

Patent

active

058044000

ABSTRACT:
A rapid single step assay suitable for the detection or quantification of enzymes, in particular, hydrolases, especially, aminopeptidases and esterases. The enzymatic reaction causes the cleavage of a metal ligand labelled hydrolase substrate. The cleaved ligand alters the electrochemiluminescence of bidentate aromatic heterocyclic nitrogen-containing ligand reagent. The change in electrochemiluminescence correlates to the presence of hydrolase activity present in the sample. The assay can be performed on an IGEN Origen.RTM. Analyzer.

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