Electrochemical sensor having three layer membrane containing im

Details Electrochemical sensor having three layer membrane containing im Electrochemical sensor having three layer membrane containing im

1985-11-25

1989-01-03

Naff, David M.

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

204403, 435174, 435177, 435817, C12M 140, C12N 1102, C12N 1114, G01N 2726

Patent

active

047957077

ABSTRACT:
An electrochemical sensor is formed having a working electrode for detecting hydrogen peroxide surrounded by a cylinder portion, and with an enzymecontaining membrane at its tip. The membrane has a porous layer permeable to hydrogen peroxide between a layer containing an immobilized enzyme capable of decomposing hydrogen peroxide and a layer containing an immobilized enzyme capable of decomposing a substrate to form hydrogen peroxide. The cylinder portion is embedded in the layer containing the hydrogen peroxide decomposing enzyme and surrounds the working electrode such that the electrode is in contact with the porous layer but is not in contact with the layer containing the hydrogen peroxide decomposing enzyme. The layer containing the hydrogen peroxide forming enzyme is on a side of the porous layer opposite the electrode so as not to contact the electrode. Activity of the hydrogen peroxide decomposing enzyme is no more than one-fourth of the activity of the hydrogen peroxide forming enzyme. The electrochemical sensor reduces base line elevation after measurement action has been discontinued and measurement of a next sample is restarted.

REFERENCES:
patent: 4172765 (1979-10-01), Keyes
patent: 4240889 (1980-12-01), Yoda et al.
patent: 4307195 (1981-12-01), Karasama et al.
patent: 4460686 (1984-07-01), Hartmeier
patent: 4579642 (1986-04-01), Niiyama et al.
patent: 4581336 (1986-04-01), Malloy et al.
Therenot et al., Analytical Chemistry, vol. 51, No. 1, 1979, pp. 96-100.

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