Electrochemical sensor having an immobilized enzyme membrane

Details Electrochemical sensor having an immobilized enzyme membrane Electrochemical sensor having an immobilized enzyme membrane

1984-11-07

1986-04-01

Kaplan, G. L.

Chemistry: electrical and wave energy

Apparatus

Electrolytic

435291, 435817, C12Q 100, C12Q 158, G01N 2730

Patent

active

045796424

ABSTRACT:
A recess of a predetermined thickness is formed in the bottom of a cylindrical vessel and a hole for the liquid-junction is formed at the center of the recess, to which is joined a resilient NH.sub.4.sup.+ selective membrane. Since the NH.sub.4.sup.+ selective membrane has a thickness larger by about 0.1 mm than the depth of the recess, the NH.sub.4.sup.+ selective membrane is protruded out of the bottom of the vessel by so much. A polyester woven fabric on which urease is immobilized is attached to the vessel so as to cover the NH.sub.4.sup.+ selective membrane. Since the immobilized enzyme membrane is applied with an appropriate tension by the slightly protruded ion selective membrane, bondability between the two membranes can be improved.

REFERENCES:
patent: 4224125 (1980-09-01), Nakamura et al.
patent: 4476005 (1984-10-01), Tokinaga et al.
G. G. Guilbault et al., Anal. Chem., vol. 45, No. 2, pp. 417-419, (1973).

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