Electrochemical method to measure DNA attachment to an...

Details Electrochemical method to measure DNA attachment to an... Electrochemical method to measure DNA attachment to an...

2007-08-08

2009-11-10

Riley, Jezia (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S005000, C435S091100, C435S091200, C536S023100, C536S024300, C536S026600

Reexamination Certificate

active

07615348

ABSTRACT:
The present disclosure provides methods and compositions for conducting an assay to detect a polynucleotide. In particular, ruthenium complexes having reduction potentials that do not coincide with the reduction potential of molecular oxygen are disclosed and amperometric techniques for their use are described. In preferred embodiments, the ruthenium complex is ruthenium (III) pentaamine pyridine and the polynucleotide that is detected is DNA. Further, techniques for enhancing detectable contrast between hybridized and unhybridized nucleic acids are disclosed. In particular, the use of elongated target strands as well as the use of uncharged probe strands are discussed.

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Steel et al. Analytical Chemistry, vol. 70, No. 22, 1998, pp. 4670-4677.

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