Electrochemical immunoassay methods

Chemistry: electrical and wave energy – Processes and products – Processes of treating materials by wave energy

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204403, 204418, 435 71, 435 79, 435 793, 435 794, 435288, 435291, 435817, 436501, G01N 2726

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active

053912721

ABSTRACT:
An electro-immunosensor which can be used to determine the presence of a wide range of analytes in solution is described. Also described are methods of determining subnanogram levels of analytes in a one-step procedure. This novel immunosensor comprises an analyte binding agent bound to an electrode and a analyte/enzyme conjugate bound to the analyte binding agent as part of a catalytic electrical circuit. Displacement of the conjugate by analyte causes a proportional decrease in current. The immunosensor may also be adapted to detect two analytes in a single step by using different enzymes conjugated to the analyte binding agents.

REFERENCES:
patent: 4318707 (1982-03-01), Litman et al.
patent: 4340448 (1982-07-01), Schiller et al.
patent: 4391904 (1983-07-01), Litman et al.
patent: 4444880 (1984-04-01), Tom
patent: 4501692 (1985-02-01), Gibbons et al.
patent: 4564598 (1986-01-01), Briggs
patent: 4663278 (1987-05-01), DiNello
patent: 4687735 (1987-08-01), DiNello et al.
patent: 4820399 (1989-04-01), Senda et al.
patent: 4970145 (1990-11-01), Bennetto et al.
patent: 4981785 (1991-01-01), Nayak
patent: 5082550 (1992-01-01), Rishpon et al.
patent: 5082786 (1992-01-01), Nakamoto
patent: 5126034 (1992-06-01), Carter et al.
patent: 5158869 (1992-10-01), Pouletty et al.
patent: 5225064 (1993-07-01), Henkens et al.
Albery et al., "Inhibited Enzyme Electrodes. Part 3. A Sensor for Low Levels of H.sub.2 S and HCN," Biosensors & Bioelectronics, 5:397-413, 1990.
Armstrong, Fraser A., "Voltametry of Metal Centres in Proteins," Perspectives on Bioinorganic Chemistry, 1:141-165, 1991.
Armstrong, F. A., and Lannon, A. M., "Fast Interfacial Electron Transfer between Cytochrome c Peroxidase and Graphite Electrodes Promoted by Aminoglycosides: Novel Electroenzymic Catalysis of H.sub.2 O.sub.2 Reduction," J. Am. Chem. Soc., 109:7211-7212, 1987.
Rowden, E. F., and Hawkridge, F. M., "Interfacial Electrochemistry of Cytochrome c At Tin Oxide, Indium Oxide, Gold, and Platinum Electrodes," J. Electroanal. Chem., 161:355-376, 1984.
Cass, A. E. G., ed., Biosensors: A Practical Approach, Oxford University Press, Oxford/New York, 1990.
Crumbliss, A. L. et al., "Amperometric Glucose Sensor Fabricated from Glucose Oxidase and a Mediator Co-Immobilized on a Colloidal Gold Hydrogel Electrode," Abstract for the ACS North Carolina Divisional Meeting, University of North Carolina at Chapel Hill, Sep. 7-9, 1989, published in USA.
Crumbliss, A. L. et al., "The Use of Inorganic Materials to Control or Maintain Immobilized Enzyme Activity," Symposium on opportunities for inorganic chemistry in biotechnology, Abstract for the ACS National Meeting in Boston, Apr. 23, 1990, published in USA.
Crumbliss, A. L. et., "The Influence of Colloidal Gold Surfaces on Enzyme Activity," Abstract for the ACS North Carolina Divisional Meeting, Sep., 1988, published in USA.
Crumbliss, A. L. et al., "Catalytic and Electroactivity of Irreversibly Adsorbed Enzymes at Gold Electrode Surfaces," Symposium on Protein Electrochemistry: Abstract for the ACS Southeast Regional Meeting (SERM), Oct., 1989, published in USA.
Fair, B. D., and Jamieson, A. M., "Studies of Protein Adsorption on Polystyrene Latex Surfaces," J. Colloid Interface Sci., 77(2):525-534, 1980.
Frew, J. E. et al., "A Method for Estimation of Hydrogen Peroxide Based On Medicated Electron Transfer Reactions of Peroxidases at Electrodes," Electroanal. Chem., 201:1, 1986.
Govindaraju, K. et al., "Active Site Chemistry of Lysyl Oxidase," J. Inorg. Biochem., 29:111, 1987.
Gregg, B. A., and Heller, A., "Cross-Linked Redox Gels containing Glucose Oxidase for Amperometric Biosensor Applications," Anal. Chem., 62:258-263, 1990.
Guo, L-H et al., "Direct Electrochemistry of Proteins and Enzymes," Adv. Inorg. Chem., 36:341-375, 1991.
Guo, L-H et al., "Direct Voltametry of the Chromatium vinosum Enzyme, Sulfide: Cytochrome c Oxidoreductase (Flavocytochrome c552)," J. Biol. Chem., 265(4):1958-1963, 1990.
Guo, L-H et al., "Direct Un-Mediated Electrochemistry of the Enzyme p-Cresolmethylhydroxylase," J. Electroanal. Chem., 266:379-396, 1989.
Hagen, W. R., "Direct Electron Transfer of Redox Proteins at the Bare glassy Carbon Electrode," J. Biochem., 182:523-530, 1989.
Hale, P. D., et al., "Amperometric Glucose Biosensors Based on Redox Polymer-Mediated Electron Transfer," Anal. Chem., 63:677-682, 1991.
Henkens, R. W. et al., "Bioactive Electrodes Using Metallo-Proteins Attached to Colloidal Gold," Rec. Trav. Chem. Pays Bas., 106:6-7, 1987.
Henkens, R. W. et al., "Biosensor Electrodes Using Colloidal Gold Supported Oxidase Enzymes," J. Inorg. Biochem., 43:120, 1991.
Henkens, R. W., and O'Daly, J. P., "Multi-Analyte Enzyme Electrodes for Environmental Monitoring," Abstract for the Proceedings of 5th International Biotechnology Conference in Copenhagen, Jul. 8-13, 1990.
Jonsson, G., and Gorton, L., "An Electrochemical Sensor for Hydrogen Peroxide Based on Peroxidase Adsorbed on a Spectrographic Graphite Electrode," Electroanalysis, 1:465-468, 1989.
Kinoshita, Kim, "Carbon: Electrochemical and Physicochemical Properties," John Wiley & Sons, New York, 1988.
Paddock, R. M., and Bowden, E. F., "Electrocatalytic Reduction of Hydrogen Peroxide via Direct Electron Transfer from Pyrolytic Graphite Electrodes to Irreversibly Adsorbed Cytochrome c Peroxidase," J. Electroanal. Chem., 260:487-494, 1989.
Zhao et al., "Direct Electron Transfer at Horseradish Peroxidase-Colloidal Golf Modified Electrodes," J. Electronanal. Chem., 327:109-119, 1992.
Stonehuerner et al., "Comparison of colloidal gold electrode fabrication methods: the preparation of a horseradish peroxidase enzyme electrode," Biochem. Methods, 117:208148, 1992.
Aizawa, M., "Principles and applications of electrochemical and optical biosensors," Analytica Chimica Acta., 250:249-256 (1991).
Huet, D. and Bourdillon, C., "Automatic apparatus for heterogeneous enzyme immunoassays based on electrocatalytic detection of the enzyme and electrochemical regeneration of the solid phase," Analytica Chimica Acta., 272:205-212 (1993).
Le Gal La Salle, A. et al., "Utilization of a Nafion.RTM.-modified electrode in a competitive homogeneous electrochemical immunoassay involving a redox cationic labelled hapten-phenytoin," J. Electroanal. Chem., 350:329-335 (1993).
Monroe, D., "Amperometric Immunoassay," Critical Reviews in Clinical Laboratory Sciences, 28(1):1-18 (1990).
Monroe, D., "Potentiometric (Bioselective Electrodes) Assay Systems: Utility and Limitations," Critical Reviews in Clinical Laboratory Sciences, 27(2):109-158 (1989).
Nakamura, R. M., et al., "Enzyme immunoassays: heterogeneous and homogeneous systems," Handbook of Experimental Immunology in Four Volumes, vol. 1: Immunochemistry, Fourth Ed., Chapter 27 (Jan. 1987).
Ngo, T. T., "Immunoassay," Current Opionion in Biotechnology, (Reprinted from vol. 2, 1991) 2:102-109 (1991).
O'Daly, J. P., et al., "Electrochemical enzyme immunoassay for detection of toxic substrances," Enzyme Microb. Technol., 14:299-302 (Apr. 1992).
Palmer, D. A., et al., "Flow Injection Electrochemical Enzyme Immunoassay for Theophylline Using a Protein A Immunoreactor and p-Aminophenyl Phosphate-p-Aminophenol as the Detection System," Analyst, 117:1679-1682 (1992).
Parra, H. J., et al., "Differential Electroimmunoassay of Human LpA-I Lipoprotein Particles on Ready-to-Use Plates," Clin. Chem., 36(8):1431-1435 (1990).
Roe, R. M., "Enzyme-Linked Immunosorbent Assay of Small Molecular Weight Toxicants," Revs. in Pesticide Toxicology, 1:273-287 (1991).
Rosen, I., and Rishpon, J., "Alkaline phosphatase as a label for a heterogeneous immunoelectrochemical sensor," J. Electroanal. Chem., 258-27-39 (1989).
Tie, Feng et al., "An improved ELISA with linear sweep voltametry detection," J. of Immuno. Methods, 149:115-120 (1992).
Yim, H. S., et al., "Polymer membrane-based ion-, gas- and bio-selective potentiometric sensors," Biosensors & Bioelectronics, 8:1-38 (1993).

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