Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-06-20
1999-02-16
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 435 915, 435 9151, 935 6, 935 17, 935 77, 935 78, C12Q 168, C12P 1934
Patent
active
058719180
ABSTRACT:
A method of detecting a nucleic acid (e.g., DNA, RNA) that contains at least one preselected base (e.g., adenine, guanine, 6-mercaptoguanine, 8-oxo-guanine, and 8-oxo-adenine) comprises (a) reacting the nucleic acid with a transition metal complex capable of oxidizing the preselected base in an oxidation-reduction reaction; (b) detecting the oxidation-reduction reaction; and (c) determining the presence or absence of the nucleic acid from the detected oxidation-reduction reaction at the preselected base. The method may be used in a variety of applications, including DNA sequencing, diagnostic assays, and quantitative analysis.
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Johnston Dean H.
Kim Jin-heung
Loomis Carson R.
Napier Mary E.
Sistare Mark F.
Jones W. Gary
Shoemaker Debra
The University of North Carolina at Chapel Hill
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