Electrochemical denaturation of double-stranded nucleic acid

Details Electrochemical denaturation of double-stranded nucleic acid Electrochemical denaturation of double-stranded nucleic acid

1996-04-01

1998-10-20

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 436 94, C12Q 168, C12P 1934

Patent

active

058244778

ABSTRACT:
A process is described for denaturing native double-stranded nucleic acid material into its individual strands in an electrochemical cell. The process disclosed is an electrical treatment of the nucleic acid with a voltage applied to the nucleic acid material by an electrode. The process may also employ a promoter compound such as methyl viologen to speed denaturation. The process may be used in the detection of nucleic acid by hybridizing with a labelled probe or in the amplification of DNA by a polymerase chain reaction or ligase chain reaction.

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