Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving glucose or galactose
Patent
1986-02-21
1989-04-11
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving glucose or galactose
435 4, 435 15, 435817, 435 25, 436 67, 2605023, 556 7, C12Q 154, C12Q 148, G01N 3372, C07F 902
Patent
active
048206369
ABSTRACT:
The invention disclosed relates to an electrochemical assay which is particularly concerned with an assay for the presence of, or amount of, glycosylated haemoglobin in a blood sample, but which extends to a general assay for detecting the presence of, monitoring the level of or determining the concentration of compounds containing cis-diol groups, such as glycols, nucleic acid components, sugars, polyols, catechols and glycosylated proteins.
When ferrocene boronic acid or a derivative thereof is present free in solution, it can act as a mediator for a wide range of oxido-reductases. However in the presence of, for example, sugars containing the cis-diol structure, the metallocene (such as ferrocene boronic acid) covalently binds to the sugar. When the ferrocene boronic acid is thus bound, it is essentially immobilized and its properties as a mediator and ability to diffuse will be markedly affected.
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"GlyoTest Kits", Pierce Chemical Company, Rockford, Ill.
"Glyco Gel", Pierce Chemical Company, Rockford, Ill.
Green Monika J.
Hill Hugh A. O.
Graeter Janelle
MediSense, Inc.
Rosen Sam
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