Electrochemical analyte sensors using thermostable peroxidase

Details Electrochemical analyte sensors using thermostable peroxidase Electrochemical analyte sensors using thermostable peroxidase

1997-02-11

1999-10-26

Beisner, William H.

Electrolysis: processes, compositions used therein, and methods

Electrolytic analysis or testing

Involving enzyme or micro-organism

204403, 435817, G01N 2726

Patent

active

059721995

ABSTRACT:
A sensor for the detection and measurement of an analyte in a biofluid. The sensor includes two enzymes. One type of sensor measures the concentration of hydrogen peroxide using a thermostable peroxidase enzyme that is immobilized in a redox hydrogel to form a sensing layer on a working electrode. This sensor also includes a hydrogen peroxide-generating second enzyme which is insulated from the redox hydrogel and electrode. This second enzyme generates hydrogen peroxide in response to the presence of an analyte or analyte-generated compound. The second enzyme may be insulated from the electrode by placement of an electrically insulating layer between the sensing layer and the second enzyme layer.

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