Electrocatalytic nucleic acid hybridization detection

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C536S023100, C536S024300

Reexamination Certificate

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07741033

ABSTRACT:
The detection of specific nucleic acid sequences using electrochemical readout would permit the rapid and inexpensive detection and identification of bacterial pathogens and the analysis of human genes. A new assay developed for this purpose is described that harnesses an electrocatalytic process to monitor nucleic acid hybridization. Furthermore, the new assay when used on nanoscale electrodes, provides ultrasensitive detection of nucleic acids.

REFERENCES:
patent: 5312527 (1994-05-01), Mikkelsen et al.
patent: 5968745 (1999-10-01), Thorp et al.
patent: 5972692 (1999-10-01), Hashimoto et al.
patent: 6123819 (2000-09-01), Peeters
patent: 6180346 (2001-01-01), Thorp et al.
patent: 6221586 (2001-04-01), Barton et al.
patent: 6325904 (2001-12-01), Peeters
patent: 6361951 (2002-03-01), Thorp et al.
patent: 6399303 (2002-06-01), Connolly
patent: 6479240 (2002-11-01), Kayyem et al.
patent: 6593090 (2003-07-01), Connolly
patent: 7361470 (2008-04-01), Kelley et al.
patent: 7361471 (2008-04-01), Gerdes et al.
patent: 2002/0081588 (2002-06-01), De Lumley-woodyear et al.
patent: 2002/0084410 (2002-07-01), Colbert et al.
patent: 2002/0158342 (2002-10-01), Tuominen et al.
patent: 2003/0087277 (2003-05-01), Fritzsche et al.
patent: 2003/0089899 (2003-05-01), Lieber et al.
patent: 2003/0143571 (2003-07-01), Sharp et al.
patent: 2003/0211637 (2003-11-01), Schoeniger et al.
patent: 2004/0040840 (2004-03-01), Mao et al.
patent: 2004/0072263 (2004-04-01), Link et al.
patent: 2004/0106203 (2004-06-01), Stasiak et al.
patent: 2004/0114445 (2004-06-01), Occhipinti et al.
patent: 2004/0136866 (2004-07-01), Pontis et al.
patent: 2009/0270266 (2009-10-01), Kelley et al.
patent: 758 063 (2003-03-01), None
patent: 0 564 254 (1993-10-01), None
patent: WO 96/06946 (1996-03-01), None
patent: WO 99/67628 (1999-12-01), None
patent: WO 02/074988 (2002-09-01), None
patent: WO 02/079514 (2002-10-01), None
patent: WO 2004/027093 (2004-04-01), None
Kelley et al; Single-base mismatch detection based on charge transduction through DNA, 1999, Nucleic Acids Res., 27, 4830-4837.
Martin CR et al, Nanomaterials in analytical chemistry, 1998, Analytical Chemistry News & Features, pp. 322A-327 A.
Ueno et al, Fabrication and electrochemical characterization of interdigitated nanoelectrode arrays, 2205, Electrochemistry Communications, 7, 161-165.
Rogers et al , Using an elestomeric phasemask for sub-100 nm photolithography in the optical near field, 1997, Appl. Phys. Lett. 70, 2658-2660.
Lee, Tris 2,2′ bipyridyl ruthenium electrogenerated chemiluminscence in analytical science, Mikrochim. Acta, 1997, 127, 19-39.
Maruyama et al, Detection of target DNA by electrochemical method, 2001, Sensors and Actuators B, 76, 215-219.
Vercoutere and Akeson; “Biosensors for DNA sequence detection”;Current Op. in Chem. Biol.; (2002); 6: 816-822.
Millan, et al.; “Sequence-Selective Biosensor for DNA Based on Electroactive Hybridization Indicators”;Anal. Chem.; (1993); 65: 2317-2323.
Hashimoto, et al.; “Sequence-Specific Gene Detection with a Gold Electrode Modified with DNA Probes and an Electrochemically Active Dye”;Anal. Chem.; (1994); 66: 3830-3833.
Hashimoto, et al.; “Novel DNA sensor for electrochemical gene detection”;Anal. Chem.; (1994); 286: 219-224.
Xu, et al.; “Immobilization of DNA on an Aluminum(III) Alkanebisphosphonate Thin Film with Electrogenerated Chemiluminescent Detection”;J. Am. Chem. Soc.; (1994); 116: 8386-8387.
Liu, et al., “Voltammetric determination of sequence-specific DNA by electroactive intercalator on praphite electrode”;Anal. Chem.; (1996); 335: 239-243.
Napier, et al.; “Probing Biomolecule Recognition with Electron Transfer: Electrochemical Sensors for DNA Hybridization”;Bioconj. Chem.; (1997); 8: 906-913.
Ropp, et al.; “Site-sleective electron transfer form purines to electrocatalysts: voltammetric detection of a biologically relevant deletion in hybridized DNA duplexes”;Chem. Biol.; (1999); 6: 599-605.
Boon, et al.; Mutation detection by electrocatalysis at DNA-modified electrodes;Nat. Biotech.; (2000); 18: 1096-1100.
Armistead, et al.; “Electrochemical Detection of Gene Expression in Tumor Samples: Overexpression of Rak Nuclear Tyrosine Kinase”; (2002);Bioconj. Chem.; (2002); 13: 172-176.
Palecek, et al.; “Electrochemical biosensors for DNA hybridization and DNA damage”;Biosens. Bioelectron.; (1998); 13: 621-628.
Thorp, H. Holden; “Cutting out the middleman: DNA biosensors based on electrochemical oxidation”;Trends in Biotechnology; (1998); 16: 117-121.
Steel, et al.; “Electrochemical Quantitation of DNA Immobilized on Gold”;Anal. Chem.; (1998); 70: 4670-4677.
Kelley, et al.; Single-base mismatch detection based on charge transduction through DNA;Nucleic Acids Research; (1999); 27(24): 4830-4837.
Wang, Joseph; “Survey and Summary from DNA biosensors to gene chips”;Nucleic Acids Research; (2000); 28(16): 3011-3016.
International Search Report based on PCT/US04/14788 dated Jun. 24, 2005.
Coche-Guerente et al., Amplification of amperometric biosensor responses by electrochemical substrate recycling. 3. Theoretical and experimental study of the phenol-polyphenol oxidase system immobilized in Laponite hydrogels and layer-by-layer self-assembled structures. Anal Chem. Jul. 15, 2001;73(14):3206-18.
Beaucage et al., Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis. Tetrahedron Lett. 1981;22(20):1859-62.
Blaser,Helicobacter pyloriand the pathogenesis of gastroduodenal inflammation. J Infect Dis. Apr. 1990;161(4):626-33. Review.
Cheng et al., Ultramicroelectrode ensembles. Comparison of experimental and theoretical responses and evaluation of electroanalytical detection limits. Anal Chem. 1989;61(7):762-6.
Finot et al., Performance of interdigitated nanoelectrodes for electrochemical DNA biosensor. Ultramicroscopy. Oct.-Nov. 2003;97(1-4):441-9.
Gasparac et al., Ultrasensitive electrocatalytic DNA detection at two- and three-dimensional nanoelectrodes. J Am Chem Soc. Oct. 6, 2004;126(39):12270-1.
Heaton et al., Electrostatic surface lasmon resonance: direct electric field-induced hybridization and denaturation in monolayer nucleic acid films and label-free discrimination of base mismatches. Proc Natl Acad Sci U S A. Mar. 27, 2001;98(7):3701-4. Epub Mar. 20, 2001.
Li et al., Carbon nanotube nanoelectrode array for ultrasensitive DNA detection. Nanoletters. 2003;3:597-602.
Li et al., Fabrication approach for molecular memory arrays. Appl Phys Lett. Jan. 27, 2003;82(4):645-7.
Li et al., Highly-ordered carbon nanotube arrays for electronics applications. Appl Phys Lett. 1999;75:367.
Malaquin et al., Nanoelectrode-based devices for electrical biodetection in liquid solution. Microelect Eng. Jun. 2004;73-74:887-92.
Mirkin et al., A DNA-based method for rationally assembling nanoparticles into macroscopic materials. Nature. Aug. 15, 1996;382(6592):607-9.
Nelson et al., Label-free detection of 16S ribosomal RNA hybridization on reusable DNA arrays using surface plasmon resonance imaging. Environ Microbiol. Nov. 2002;4(11):735-43.
Nelson et al., Surface plasmon resonance imaging measurements of DNA and RNA hybridization adsorption onto DNA microarrays. Anal Chem. Jan. 1, 2001;73(1):1-7.
Peterson et al., Hybridization of mismatched or partially matched DNA at surfaces. J Am Chem Soc. Dec. 11, 2002;124(49):14601-7.
Peterson et al., The effect of surface probe density on DNA hybridization. Nucleic Acids Res. Dec. 15, 2001;29(24):5163-8.
Pividori et al., Electrochemical genosensor design: immobilisation of oligonucleotides onto transducer surfaces and detection methods. Biosens Bioelectron. Aug. 2000;15(5-6):291-303.
Shi, Technologies for individual genotyping: detection of genetic polymorphisms in drug targets and disease genes. Am J Pharmacogenomics. 2002;2(3):197-205. Review.
Smalley, Chip senses trace DNA. Jul. 30/Aug. 6, 2003. TRN Mag.com. 2 pages.
Smith et al., Theory of the

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