Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1987-05-13
1989-06-20
Niebling, John F.
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
2041828, 204299R, G01N 2728, G01N 2726
Patent
active
048407143
ABSTRACT:
An apparatus especially suitable for transferring a particular specimen from a polyacrylamide electrophoresis or like gel onto a support sheet by means of electroblotting is disclosed herein along with its method of operation. This apparatus utilizes a pair of plate-shaped electrode assemblies, each of which in the disclosed embodiment is a multilayered assembly including an electrode layer of electrically conductive material and a layer of thermally conductive material which serves as a heat sink. These electrode assemblies are adjustably supported in spaced-apart but confronting relationship to one another with the gel and support sheet sandwiched therebetween. At the same time, the electrode layers are connected to a source of power for producing an electric field across the gel and support sheet in order to transfer the specimen from the gel to the sheet by means of electroblotting. In the specific embodiment disclosed, the spacing between the electrode assemblies can be adjustably decreased at a number of different points in order to ensure that they remain parallel to one another in their adjusted positions whereby to squeeze the gel and support sheet uniformly along the extend of the gel.
REFERENCES:
patent: 4452901 (1984-06-01), Gordon et al.
patent: 4541910 (1985-09-01), Davis III, et al.
patent: 4589965 (1986-05-01), Kreisher
patent: 4622124 (1986-11-01), Kreisher et al.
American Bionetics, Inc.
Niebling John F.
Starsiak Jr. John S.
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