Electro-optical system for use in evaluating immunological react

Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Means for analyzing liquid or solid sample

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73 641, 356338, 422 58, 422102, G01N 2151, G01N 3316

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active

044003536

ABSTRACT:
The method of this invention comprises introducing the fluid biological test specimen and the reagent (e.g., latex spheres) into a reaction zone in the image cell, evenly mixing and incubating the mixture thereby forming agglutinated particles. After transilluminating the reaction zone and imaging the light beams transmitted therethrough on the image sensor, the dark areas formed on the surface of the image sensor are measured, preferably electronically. This procedure is repeated for a reference specimen and the total dark imaged area is compared with the total dark area obtained for the unknown specimen for qualitative determination.
In order to obtain the concentration of the immunoreactive component in the unknown specimen, the foregoing procedure is repeated for at least two specimens with known antibody concentrations, one of which may be a negative control. The concentration of the immunoreactive component of the unknown sample is then determined from the total dark imaged area thereof and the relationship between the total dark imaged areas of the known specimen and their respective concentrations.

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