Electro-optical apparatus for microbial identification and enume

Details Electro-optical apparatus for microbial identification and enume Electro-optical apparatus for microbial identification and enume

1984-07-11

1986-03-18

Naff, David M.

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

435 32, 435 34, 435 39, 435291, 435808, C12M 136, C12Q 118, C12Q 104, C12Q 106

Patent

active

045769168

ABSTRACT:
Rapid and accurate identifying and enumerating of microorganisms is carried out with an apparatus having an electrical cell, an optical sensing assembly, an automated microbial sample delivery means and an electrical signal processing means. The electrical cell contains a chamber having an inlet and outlet and paired electrodes located adjacent walls of the chamber. The optical sensing assembly has a laser light source to pass a beam of laser light through the electrical cell and between the electrodes, and a laser light detector in alignment with the light source to receive laser light emerging from the electrical cell. The electrical signal processing means translates variations in birefringence caused by a microbial sample into quantitative data. In operation of the apparatus, a polarized beam of laser light passes through a microbial sample containing an inhibiting reagent while the sample is in an electrical field between the electrodes, and the extend of birefringence is measured.

REFERENCES:
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patent: 4101383 (1978-07-01), Wyatt et al.
patent: 4252897 (1981-02-01), Axford et al.
patent: 4395397 (1983-07-01), Shapiro
Morris, et al., Biochimica et Biophysica Acta, vol. 392, 1975, pp. 328-334.
Morris, et al., Biochimica et Biophysica Acta, vol. 497, 1977, pp. 253-259.

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