Electro insertion of proteins into red cell membranes

Details Electro insertion of proteins into red cell membranes Electro insertion of proteins into red cell membranes

1991-12-17

1993-08-17

Naff, David M.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Cell membrane or cell surface is target

514 8, C12N 1300, A61K 3710

Patent

active

052368351

ABSTRACT:
A method of incorporating human CD4 or glycophorin into a red blood cell membrane is provided comprising exposing the red blood cell to an electric field while the cell is suspended in an electro-insertion medium in the presence of a buffered solution (suspension) of the proteins. These proteins bear a hydrophobic membrane spanning sequence. The resultant cell of the electro-insertion is then contacted with a resealing medium.

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Biological Abstracts, vol. 80, No. 1, 1985, abstract No. 622, E. H. Serpersu et al.; Reversibel and irreversible modification of erythrocyte membrane permeability by electric field.
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