Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1996-12-09
1999-02-02
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
204403, 435 25, 435174, 4352871, 435817, 534607, C12Q 132, G01N 2726, C12M 100, C09B 4410
Patent
active
058663533
ABSTRACT:
A variety of diazacyanine mediators that are soluble in aqueous media and which do not inhibit enzymatic activity are provided for use on the surface of a working electrode of a electrochemical biosensor for electrochemical co-enzyme regeneration. The co-enzyme, dihydronicotinamide adenine dinucleotide (NADH) or dihydronicotinamide adenine dinucleotide phosphate (NADPH), is oxidized to NAD.sup.+ OR NADP.sup.+ which is reduced by an oxidoreductase such as a dehydrogenase acting on a substrate. By applying the mediator together with NADH or NADPH to the surface of the working electrode, the voltage necessary to achieve oxidation is substantially reduced. Biosensor electrodes such as graphite electrodes may be produced-by screen printing techniques.
REFERENCES:
patent: 4710570 (1987-12-01), Thien
patent: 5208325 (1993-05-01), Berneth et al.
patent: 5436323 (1995-07-01), Berneth et al.
patent: 5498542 (1996-03-01), Corey et al.
patent: 5520786 (1996-05-01), Bloczynski et al.
Berneth Horst
Bocker Thomas
Giera Henry
Murray Alison J.
Siegmund Hans-Ulrich
Bayer Corporation
Jeffers Jerome L.
Naff David M.
LandOfFree
Electro chemical biosensor containing diazacyanine mediator for does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Electro chemical biosensor containing diazacyanine mediator for , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Electro chemical biosensor containing diazacyanine mediator for will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1116282