Electro-blotting of electrophoretically resolved fluroescent-lab

Details Electro-blotting of electrophoretically resolved fluroescent-lab Electro-blotting of electrophoretically resolved fluroescent-lab

1990-02-16

1991-05-28

Niebling, John F.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

2041801, 2041828, 204299R, 536127, C07H 106, C25B 700

Patent

active

050192310

ABSTRACT:
Saccharide in mixtures are separated into groups or individual saccharides and tested for their affinity for particular proteins. The method of the invention is carried out by conjugating saccharides in a mixture with 4-amino-1-naphthalene sulfonic acid (ANSA) to form conjugates. The saccharide/ANSA conjugates are subjected to electrophoretic resolution within gels. The resolved bands of material are electro-blotted onto charged nylon membranes to provide a stable record of the electrophoretic separation. The blots on the nylon membranes are brought into contact with particular proteins in order to determine the binding affinity of these particular proteins to particular saccharides on the nylon membrane. The proteins are preferably bound to labels so that the binding of the proteins to the saccharides can be easily detected.

REFERENCES:
patent: 4109075 (1978-08-01), Deaton
patent: 4305799 (1981-12-01), Schwarz et al.
patent: 4666581 (1987-05-01), Itoh et al.

Affiliated with

Also associated with

Rating

Electro-blotting of electrophoretically resolved fluroescent-lab does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Electro-blotting of electrophoretically resolved fluroescent-lab, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Electro-blotting of electrophoretically resolved fluroescent-lab will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-34135