Electro-blotting of electrophoretically resolved fluorescent-lab

Details Electro-blotting of electrophoretically resolved fluorescent-lab Electro-blotting of electrophoretically resolved fluorescent-lab

1990-09-27

1992-03-10

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

2041828, 204299R, 536127, G01N 2726

Patent

active

050947311

ABSTRACT:
Saccharide in mixtures are separated into groups or individual saccharides and tested for their affinity for particular proteins. The method of the invention is carried out by conjugating saccharides in a mixture with charge-generating moieties and moieties capable of fluorescing such as 4-amino-1-naphthalene sulfonic acid (ANSA) to form conjugates. The saccharide conjugates are subjected to electrophoretic resolution within gels. The resolved bands of material can be seen under ultraviolet light and are electro-blotted onto charged nylon membranes to provide a stable record of the electrophoretic separation. The blots (visible under ultraviolet light) on the nylon membranes are brought into contact with particular proteins in order to determine the binding affinity of these particular proteins to particular saccharides on the nylon membrane. The proteins are preferably bound to labels so that the binding of the proteins to the saccharides can be easily detected.

REFERENCES:
patent: 4109075 (1978-08-01), Deaton
patent: 4305799 (1981-12-01), Scharz et al.
patent: 4666581 (1987-05-01), Itoh et al.

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