Electric field amplified oligonucleotide ligase analysis

Details Electric field amplified oligonucleotide ligase analysis Electric field amplified oligonucleotide ligase analysis

1997-10-23

2000-02-29

Myers, Carla J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435 915, 435 9152, C12Q 168, C12P 1934

Patent

active

060307811

ABSTRACT:
An electric field amplified oligonucleotide ligase analysis includes combining a plurality of oligonucleotide probes carried on a support at a binding site with a solution including a target molecule, a plurality of oligonucleotide segments including a marker and ligase. A first electric field surrounding the plurality of oligonucleotide probes is generated to attract the target molecule to a first of the plurality of oligonucleotide probes to which it hybridizes. A first of the plurality of oligonucleotide segments also hybridizes with the target molecule and covalently bonds to the first oligonucleotide probe due to the presence of ligase in the solution. The target molecule is separated by generating a second electric field surrounding the plurality of oligonucleotide probes. The generation of the electric fields are alternately repeated to amplify the hybridization signal.

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Dubiley et al (1997). Fractionation, phosphorylation, and ligation on oligonucleotide microchips to enhance sequencing by hybridization. Nucleic Acids Research 25:2259-2265, Jun. 1997.

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