Elastomeric device for cell seeding on the bottom of a filter

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Solid support and method of culturing cells on said solid...

Reexamination Certificate

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Details

C435S401000, C435S373000, C422S105000, C436S177000

Reexamination Certificate

active

07879607

ABSTRACT:
An article is provided herein for use in seeding cells on at least one filter extending across at least one well of an assay device. The article includes an elastomeric body having spaced-apart first and second surfaces, and at least one channel extending between, and through, the first and second surfaces. The channel is formed to sealingly, and detachably, engage an outer surface of the well with the filter being at least partially encompassed by the channel. Advantageously, with the subject invention, a cell monolayer can be formed on the exterior surface of the filter. The assay device may be a multiwell plate, an insert plate, a column, a test tube, or a pipette.

REFERENCES:
patent: 5272083 (1993-12-01), Butz et al.
patent: 5468638 (1995-11-01), Barker et al.
patent: 5470743 (1995-11-01), Mussi et al.
patent: 5665596 (1997-09-01), Mussi
patent: 2003/0073228 (2003-04-01), Duffy et al.
patent: 101 17 723 (2002-10-01), None
patent: 0 735 134 (1996-10-01), None
patent: 0 757 097 (1997-02-01), None
English translation of DE 10117723 (Machine translation).

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