Drug – bio-affecting and body treating compositions – Preparations characterized by special physical form – Implant or insert
Patent
1990-04-09
1993-06-29
Patterson, Jr., Charles L.
Drug, bio-affecting and body treating compositions
Preparations characterized by special physical form
Implant or insert
424484, 43524023, 4352402, 514 21, 530353, 530382, 530383, A61K 970, A61K 3712, C12N 500
Patent
active
052234207
DESCRIPTION:
BRIEF SUMMARY
The subject of the invention is an elastin-based product, endowed, especially, with properties of elasticity and robustness.
The invention also relates to a procedure for its preparation as well as to the biological applications of this product, in particular, for the preparation of biomaterials and supporting materials which may be used, in particular, as artificial tissues or supports for cell cultures.
It is known that elastin is a constituent of the elastic components of an organism, in particular, of the lungs, skin, arterial walls and umbilical cord.
It is a protein of the scleroprotein type, generally resistant to the action of acids, alkalis and proteolytic enzymes, with the exception of elastase, for which it is a specific substrate and is degraded by it.
In vivo, elastin is protected from the action of elastase, especially by complexes between elastase and inhibitors in the serum. However, in various pathological processes of inflammatory origin or related to age, such as pulmonary emphysema or atherosclerosis, degradation of elastin, probably due to elastase, is observed from an early stage of the disease onwards.
In the course of earlier work, some of the co-inventors of the present invention demonstrated the existence in vitro of the complexes between elastase and inhibitors in the serum formed in vivo in the plasma or the serum, and have studied their capacity to bind to elastin and their elastolytic activity induced by raising the pH from 6 to 8.6.
In the course of the study of a possible elastinolytic activity in human serum or plasma, it had been reported that the adsorption of plasma or serum to elastin, when incubation was carried out at 37.degree. C. in a 0.2M acetate buffer, pH 6, which was 1 mM with respect to the acetates of calcium, magnesium and manganese, is accompanied by a visible modification of the appearance of the protein which loses its granular character and becomes flocculent.
Research designed to lead to an understanding of this phenomenon had led the inventors to the observation that, by working under certain conditions, it is possible to form and isolate a new elastin-based product endowed with remarkable properties of both strength and elasticity. Another particularly advantageous feature is its plasticity.
Thus, the subject of the invention is to furnish a new elastin-based product, the properties of which, in particular its flexibility and robustness, make it possible to prepare a biomaterial or a supporting material in various forms, especially in the form of membranes, connecting pieces or filaments, displaying satisfactory properties for various biological applications.
Another subject of the invention is to provide a procedure for the preparation of this product with the required properties under convenient working conditions.
The invention also relates to the exploitations of the properties of this product for the preparation of biomaterials and supporting materials which cater for needs of great importance, for example, for the repair of tissues or the development of supports for cell cultures.
The product according to the invention derived from elastin is characterised in that it consists of an adduct containing elastin and at least one protein of the type which can be coagulated and/or activated by thrombin.
In the context of the present invention, it is understood that the term elastin covers native or mature elastins, their derivatives and elastins modified, especially, by hydrolysis.
In an advantageous manner, a product of this type possesses a high chemical resistance and the structural properties of high elasticity and great strength. In addition, it displays great plasticity and flexibility as well as properties of impermeability and it can also be moulded, which confers on it great importance, especially for the purposes of biological applications.
In one of its forms the elastin-based product of the invention consists of an adduct containing elastin and soluble fibrin.
It will be recalled that soluble fibrin is formed as an intermediate from fibrinog
REFERENCES:
patent: 4614794 (1986-09-01), Easton et al.
Burleson, R. L., et al. (1978) J. Surg. Res. 25, 523-529.
Pool, J. G. et al. (1965) New Eng. J. Med. 273, 1443-1447.
Thornton, J. W., et al., (1984) Burn Wound Coverings 1, 147-159.
Burke, J. F. (1984) Surg. Sci. Ser. 2, 311-315.
"The Condensed Chemical Dictionary", (G. G. Hawley, ed), Van Nostrand Reinhold, New York, 1977, pp. 16 and 464.
Burleson, R., et al. (1972) Surgery 72(2), 315-322.
Burleson, R. L., et al. (1979) Chem. Abst. 90:84529y.
Thornton, J. W., et al. (1984) Chem. Abst. 101:149225p.
El-Far, M. A., et al. (1985) Chem. Abst. 103:2997z.
Rabaud, M., et al. (1986) Chem. Abst. 105:58551h.
Bricaud Henri
Lefebure Clement Francoise
Rabaud Michel
Schmidthaeusler Roland
Institut National de la Sante et de la Recherche Medicale
Patterson Jr. Charles L.
LandOfFree
Elastin-based product, a procedure for its preparation and its b does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Elastin-based product, a procedure for its preparation and its b, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Elastin-based product, a procedure for its preparation and its b will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1754730